Biomarkers for Babesia

• US 20090215086A1
• Filed: 12/12/2006
• Published: 08/27/2009
• Est. Priority Date: 12/12/2005
• Status: Abandoned Application

• Current Assignee: Brian Ward, Mark W. Duncan, Mike Edwards, Momar Ndao, Peter Krause, Terence William Spithill
• Original Assignee: Brian Ward, Mark W. Duncan, Mike Edwards, Momar Ndao, Peter Krause, Terence William Spithill
• Inventors: Krause, Peter, Edwards, Mike, Ward, Brian, Duncan, Mark W., Spithill, Terence William, Ndao, Momar
• Application Number:
  US12/097,037
• Publication Number:
  US 20090215086A1
• Time in Patent Office: Days
• US Class Current: 435/7.220
• CPC Class Codes: G01N 33/56905 Protozoa
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying babesia status in a patient. In particular, the biomarkers of this invention are useful to classify a subject sample as infected with babesia or not infected with babesia. The biomarkers can be detected by SELDI mass spectrometry.

Claims

1-88. -88. (canceled)

89. A method for qualifying babesia status in a subject comprising:

measuring at least one biomarker in a biological sample from the subject, wherein the at least one biomarker is selected from the group consisting of the biomarkers of Table 1, Table 2, and Table 3; and

correlating the measurement -with babesia status.

95. The method of any one of claims 89 to 91 or the kits of claim 92 or 94, wherein the at least one biomarker is selected from the group consisting of biomarkers of molecular masses of about 2.8, 2.9, 3, 3.1, 3.2, 3.6, 3.8, 4, 4.1, 4.2, 4.3, 4.8, 4.9, 6.4, 7, 7.1, 7.2, 7.3, 7.5, 7.7, 7.9, 8.7, 8.8, 8.9, 10, 10.1, 10.2, 10.3, 10.4, 10.9, 11, 11.2, 11.3, 11.6, 11.8, 11.9, 12.6, 12.7, 12.8, 12.9, 13, 13.1, 13.2, 13.6, 13.8, 14.1, 14.4, 14.7, 15.1, 15.6, 15.9, 16.5, 16.7, 17.3, 17.8, 21.9, 22, 22.2, 22.3, 23.5, 23.6, 25.5, 25.8, 28, 28.1, 28.2, 33, 33.1, 33.2, 33.3, 34.1, 36.1, 39.8, 43.4, 44, 44.2, 44.3, 44.8, 45.1, 46.1, 47.7, 51, 53, 53.6, 60.6, 62.4, 66.9, 79, 18.1, 19.2, 22.3, 26.5, 39.6, 39.9, 40.1, 41.3, 43.2, 43.6, 44.2, 44.4, 44.6, 45.2, 44.7, 50, 50.5, 51.2, 51.5, 51.9, 52.5, 52.7, 58.9, 59.1, 59.6, 59.8, 60.5, 61.6, 61.9, 62.3, 62.8, 64, 66.3, 66.6, 78.5, 79, 79.2, 79.5, 79.6, 99.3, 99.6, 110.2, 131.8, 133.5, 134.6, 146, 146.6, 167.8, 168, and 196.4 kDa.

96. The method of any of claims 89, 90, or 95, further comprising:

managing subject treatment based on the status.

97. The method of claim 96, further comprising:

measuring the at least one biomarker after subject management and correlating the measurement with disease progression.

90. A method for determining the course of babesia comprising:

measuring, at a first time, at least one biomarker in a biological sample from the subject, wherein the at least one biomarker is selected from the group consisting of the biomarkers of Table 1, Table 2, and Table 3;

measuring, at a second time, the at least one biomarker in a biological sample from the subject; and

comparing the first measurement and the second measurement;

wherein the comparative measurements determine the course of babesia.

91. A method comprising measuring at least one biomarker in a sample from a subject, wherein the at least one biomarker is selected from the group consisting of biomarkers of Table 1, Table 2, and Table 3.

92. A kit comprising:

a solid support comprising at least one capture reagent attached thereto, wherein the capture reagent binds at least one biomarker from a first group consisting of the biomarkers of Table 1, Table 2 and Table 3.

93. The kit of claim 92, wherein the solid support comprising a capture reagent is a SELDI probe.

94. The kit of claims 92 or 93, additionally comprising:

a container containing at least one of the biomarkers of Table 1, Table 2, or Table 3.

98. A composition comprising a purified biomolecule selected from the group consisting of the biomarkers of Table 1, Table 2, and Table 3.

99. A composition comprising a biospecific capture reagent that specifically binds a biomolecule selected from group consisting of the biomarkers of Table 1, Table 2, and Table 3.

100. A composition comprising a biospecific capture reagent bound to a biomarker of Table 1, Table 2, and Table 3.

101. A software product comprising:

a) code that accesses data attributed to a sample, the data comprising measurement of at least one biomarker in the sample, the biomarker selected from the group consisting of the biomarkers of Table 1, Table 2, and Table 3; and

b) code that executes a classification algorithm that classifies the <

disease>

status of the sample as a function of the measurement.

102. A method comprising detecting a biomarker of Table 1, Table 2, or Table 3 by mass spectrometry or immunoassay.

103. A method for identifying a compound that interacts with a biomarker of Table 1, Table 2 or Table 3 wherein said method comprises:

a) contacting a biomarker of Table 1, Table 2, or Table 3 with a test compound; and

b) determining whether the test compound interacts with a biomarker of Table 1, Table 2, or Table 3.