METHODS, SURFACE MODIFIED PLATES AND COMPOSITIONS FOR CELL ATTACHMENT, CULTIVATION AND DETACHMENT
First Claim
1. A method to enhance the attachment of cells to a surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer, comprising the steps of:
- a. Obtaining a suspension of the cells,b. Treating the suspension of cells with at least one compound selected from the group consisting of;
a compound capable of inhibiting Rho kinase activity, and a compound capable of inhibiting Rho activity, andc. Adding the suspension of cells to the surface and allowing the cells to attach.
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Accused Products
Abstract
The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
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Citations
21 Claims
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1. A method to enhance the attachment of cells to a surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer, comprising the steps of:
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a. Obtaining a suspension of the cells, b. Treating the suspension of cells with at least one compound selected from the group consisting of;
a compound capable of inhibiting Rho kinase activity, and a compound capable of inhibiting Rho activity, andc. Adding the suspension of cells to the surface and allowing the cells to attach. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method to enhance the attachment of cells to a surface containing from at least about 0.9% N, a sum of O and N of greater than or equal to 22.3% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer, comprising the steps of:
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a. Obtaining a suspension of the cells, and b. Adding the suspension of cells to the surface and allowing the cells to attach. - View Dependent Claims (11, 12, 13, 14, 15)
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- 16. A surface that is part of a vessel or matrix intended for use in cell culture or analysis, containing from at least about 0.9% N, a sum of O and N of greater than or equal to 22.3% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer, wherein the surface allows the attachment and cultivation of cells.
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19. A composition comprising:
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a. A surface that is part of a vessel or matrix intended for use in cell culture or analysis, containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer, and b. At least one compound selected from the group consisting of;
a compound capable of inhibiting Rho kinase activity, and a compound capable of inhibiting Rho activity. - View Dependent Claims (20, 21)
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Specification