SYSTEM AND METHOD OF ANALYTE DETECTION USING DIFFERENTIAL RECEPTORS

• US 20090215646A1
• Filed: 06/30/2006
• Published: 08/27/2009
• Est. Priority Date: 07/01/2005
• Status: Abandoned Application

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Wright, Aaron T., Zhong, Zhenlin, Neikirk, Dean P., Anslyn, Eric V., McDevitt, John T., Shear, Jason B.
• Application Number:
  US11/994,353
• Publication Number:
  US 20090215646A1
• Time in Patent Office: Days
• US Class Current: 506/12
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/54386 Analytical elements

Abstract

Methods and systems for detecting the presence of analytes are described. A fluid or gas sample containing one or more analytes may pass through a particle-based sensor array. Detection and analysis techniques may be applied to determine the identity and quantity of the analytes.

Claims

1-7. -7. (canceled)

8. A sensor array comprising:

one or more particles positioned in one or more cavities, wherein a particle comprises;

a polymeric resin bead; and

one or more receptors comprising one or more attracting components coupled to the polymeric resin.

9. The sensor array of claim 8, further comprising one or more light sources.

10. The sensor array of claim 8, further comprising one or more detectors optically coupled to one or more of the particles.

11. The sensor array of claim 8, wherein two or more of the attracting components are coupled to the polymeric resin by an additional attracting component.

12. The sensor array of claim 8, wherein at least one attracting component comprises a boronic acid.

13. The sensor array of claim 8, wherein at least one attracting component comprises a boronic acid on an end of the attracting component.

14. The sensor array of claim 8, wherein two or more of the attracting components comprise boronic acid positioned on an end of an attracting component.

15. The sensor array of claim 8, wherein at least one receptor is a synthetic receptor.

16. A method comprising:

passing a fluid over one or more particles comprising one or more receptors comprising two or more attracting components coupled to a polymeric resin bead;

detecting one or more signals produced by the interaction of one or more analytes in the fluid with one or more of the particles; and

assessing the pattern of one or more of the produced signals.

17. The method of claim 16, further comprising adding a visualization agent to one or more of the particles.

18. The method of claim 16, further comprising comparing the pattern of one or more of the produced signals to one or more known patterns correlating to one or more known analytes.

19. A method comprising:

passing a fluid comprising one or more analytes over one or more particles in a cavity of a sensor array, wherein a particle comprises one or more receptors comprising two or more attracting components coupled to a polymeric resin, wherein one or more of the analytes associates with two or more of the attracting components;

adding one or more visualization agents to the particles, wherein one or more of the visualization agents displace at least one analyte from the particle; and

detecting one or more signals produced by the displacement of at least one analyte from the at least one particle.

20. The method of claim 19, further comprising assessing the pattern of one or more of the produced signals.

21. The method of claim 20, further comprising assessing an identity of an analyte based on the pattern.

22. A method comprising:

passing one or more visualization agents over one or more particles in one or more cavities in a sensor array, wherein one or more of the particles comprise one or more receptors comprising two or more attracting components coupled to a polymeric resin, and wherein two or more of the attracting components associate with one or more visualization agents;

passing a fluid containing at least one analyte over the particles, wherein the particles interact with the analyte, and wherein at least one analyte displaces at least one visualization agent from the particle; and

detecting one or more signals produced by the displacement of one or more of the visualization agents from one or more of the particles.

23. The method of claim 22, further comprising assessing the pattern of one or more of the produced signals.

24. The method of claim 23, further comprising assessing the identity of an analyte based on the pattern.

25. The method of claim 22, wherein at least one visualization agent is xylenol orange or pH indicator.

27. The method of claim 22, further comprising adding Ca(II) to the particles.

28. The method of claim 22, further comprising washing particles in the sensor array after a signal is detected with a visualization agent.

29. The method of claim 22, further comprising washing particles in the sensor array after a signal is detected with an acid or a buffer.

31. The method of claim 22, further comprising washing particles in the sensor array after a signal is detected with an anionic or cationic indicator.

26. (canceled)

30. (canceled)

32. (canceled)