Optical sensor with enhanced reflectance

• US 20090219509A1
• Filed: 02/29/2008
• Published: 09/03/2009
• Est. Priority Date: 02/29/2008
• Status: Active Application

• Current Assignee: Light Pointe Medical Inc
• Original Assignee: Light Pointe Medical Inc
• Inventors: Nomura, Hiroshi
• Application Number:
  US12/072,940
• Publication Number:
  US 20090219509A1
• Time in Patent Office: Days
• US Class Current: 356/39
• CPC Class Codes: G01N 2021/7773 Reflection
• CPC Class Codes: G01N 21/55 Specular reflectivity
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/544 the carrier being organic

Abstract

A device for determination of analytes is disclosed that comprises an optical material having a deposit on a surface thereof, where this deposit includes an analyte-reactive reagent and a particulate means for enhancing reflectance of a light beam emitted through the optical material into the analyte-reactive reagent. The enhanced reflectance provides a return of light through the optical material at a level which is not only analytically useful, but involves sufficient returned light intensity so as to enable markedly improved accuracy in analytical determinations. The particulate means most preferably includes hollow spherical particles.

Claims

1. A sensor for determination of analytes, comprising an optical material with a textured surface site having a deposit thereon comprising an analyte-reactive reagent and a particulate means for enhancing reflectance of a light beam, the light beam originating through the optical material into a sampling zone of the analyte-reactive reagent.

2. The sensor of claim 1 wherein the reagent produces an optically detectable change upon reaction with at least one analyte.

3. The sensor of claim 2 wherein the analyte is blood glucose.

4. The sensor of claim 2 wherein the particulate means comprises light scattering particles

5. The sensor of claim 2 wherein the particulate means comprises hollow polymeric beads.

6. The sensor of claim 5 wherein reflectance is enhanced at least fourfold due to the presence of the particulate means.

7. The sensor of claim 1 wherein said means is simultaneously co-deposited with the analyte-reactive reagent on the textured surface.

10. The sensor of claim 1wherein the deposit has a thickness of less than 5 μ

m.

12. The sensor of claim 5 wherein light from a source is beamed through said material to said coating on said textured surface and reflectance is transmitted through said material to a detector.

8. The sensor of claim 8 wherein said means comprises light scattering particles.

9. The sensor of claim 9 wherein the particles are deposited within valleys or crevices within a textured surface formed by atomic oxygen texturing.

11. The sensor of claim 9 wherein the deposit has a thickness of less than 2 μ

m.

13. A sensor for determination of at least one analyte in a fluid comprising an optic fiber having proximal and distal ends, further having a textured surface on the distal end and having a coating comprising at least one analyte-reactive reagent disposed on the textured surface, said reagent producing an optically detectable change upon reaction with at least one analyte in a fluid to be presented to the distal end, wherein a means is provided for enhancing reflectance into said fiber from a sampling zone associated with said coating.

14. The sensor of claim 14 wherein the proximal end of said fiber receives light from a source and emanates reflectance light to a detector.

15. The sensor of claim 15 wherein said means comprises light scattering particles co-disposed with said analyte-reactive reagent on said textured surface agent.

16. The sensor of claim 16 wherein said means comprises inanimate particulate matter deposited within valleys or crevices within said textured surface.

17. The sensor of claim 16 wherein said means comprises hollow polymeric particles.

18. A device for measurement of an analyte in a fluid comprising an optical material having a deposit on a surface thereof comprising an analyte-reactive reagent and a plurality of hollow polymeric particles, a substantial portion of the reagent being disposed between the surface of the optical material and the hollow polymeric particles, wherein reflectance of a light beam emitted through the optical material into a sampling zone associated with the analyte-reactive reagent is enhanced.

19. The device of claim 19 wherein the hollow polymeric particles comprise beads having a light scattering characteristic.

20. The device of claim 19 wherein the hollow polymeric particles comprise beads of a composition transparent to visible light.

21. The device of claim 19 wherein the deposit is wettable by water and body fluids.

22. A method of analyzing a fluid sample for an analyte comprising presenting a fluid sample containing the analyte to a site on an optical material, the site having a deposit comprising an analyte-reactive reagent and a particulate means for enhancing reflectance, developing an optically detectable change in a reagent sampling zone associated with the analyte-reactive reagent, emitting a beam of light through the material into the reagent sampling zone, returning reflectance from the reagent sampling zone through the optical material to a suitable detector, and measuring the reflectance for spectral qualities correlative to the presence and quantity of the analyte.

23. The method of claim 23 wherein the site on the optical material comprises a surface textured by means of atomic oxygen texturing.

24. The method of claim 23 wherein the particulate means comprises hollow polymeric particles.

25. The method of claim 24 wherein the particulate means comprises hollow polymeric particles.