Method and System for Measuring Sub-Surface Composition of a Sample

• US 20090219523A1
• Filed: 02/23/2006
• Published: 09/03/2009
• Est. Priority Date: 09/16/2005
• Status: Active Grant

• Current Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Morris, Michael D., Schulmerich, Matthew, Finney, William F.
• Granted Patent: US 8,054,463 B2
• Time in Patent Office: Days
• US Class Current: 356/300
• CPC Class Codes: G01N 2021/656 Raman microprobe
• CPC Class Codes: G01N 21/474 Details of optical heads therefor, e.g. using optical fibres
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

In a method for measuring a composition of a sample, an illumination surface area of the sample is illuminated using a light source, and light from a plurality of emitting surface areas of the sample is received, each emitting surface area at a different location, the received light scattered by the sample. A cumulative area of the illumination surface area is greater than a cumulative area of two emitting surface areas of the plurality of emitting surface areas. For each emitting surface area, spectral content information associated with received light corresponding to that emitting surface area is determined, and composition information corresponding to a sub-surface region of the sample is determined based on the determined spectral content information. Different shapes of illumination surface areas as well as the plurality of emitting surface areas may advantageously be utilized for various specimen or sample geometries or illumination sources.

Claims

1. A method for measuring a composition of a sample, the method comprising:

irradiating an illumination surface area of the sample using a light source;

receiving light from a plurality of emitting surface areas of the sample, each emitting surface area at a different location, the received light scattered by the sample;

wherein a cumulative area of the illumination surface area is greater than a cumulative area of two emitting surface areas of the plurality of emitting surface areas;

for each emitting surface area, determining spectral content information associated with received light corresponding to that emitting surface area; and

determining composition information corresponding to a sub-surface region of the sample based on the determined spectral content information.

2. A method according to claim 1, wherein the cumulative area of the illumination surface area is substantially the same as a cumulative area of the plurality of surface areas.

3. A method according to claim 1, wherein the cumulative area of the illumination surface area is substantially less than a cumulative area of the plurality of surface areas.

4. A method according to claim 1, wherein the cumulative area of the illumination surface area is substantially greater than a cumulative area of the plurality of surface areas.

7. A method according to claim 1, wherein the cumulative area of the illumination surface area is greater than a cumulative area of five emitting surface areas of the plurality of emitting surface areas.

12. A method according to claim 1, wherein the cumulative area of the illumination surface area is greater than a cumulative area of ten emitting surface areas of the plurality of emitting surface areas.

13. A method according to claim 1, wherein the illumination surface area is one contiguous area.

14. A method according to claim 1, wherein the illumination surface area comprises a plurality of separate areas.

15. A method according to claim 1, wherein irradiating the illumination surface area comprises shaping the illumination surface area.

16. A method according to claim 15, wherein a shape of the illumination surface area comprises at least one of a circle, an oval, a square, a rectangle, a triangle, a line, an I shape, or a cross.

17. A method according to claim 1, wherein the cumulative area of the illumination surface area overlaps at least partially with at least some of the plurality of surface areas.

19. A method according to claim 1, wherein the cumulative area of the illumination surface area does not overlap with the plurality of surface areas.

23. A method according to claim 1, wherein receiving light from the plurality of emitting surface areas of the sample comprises collecting light using a plurality of optical fibers, each optical fiber corresponding to a different one of the plurality of emitting surface areas.

24. A method according to claim 1, wherein collecting light using the plurality of optical fibers comprises using a probe having optical collection fibers arranged in at least one of a circle, an oval, at least one semi-circle, at least one V shape, and at least one line.

25. A method according to claim 24, wherein collecting light using the plurality of optical fibers comprises using a probe having optical collection fibers included within an interior of a circle.

26. A method according to claim 24, wherein collecting light using the plurality of optical fibers comprises optical using a probe having optical collection fibers included only on a circumference of a circle.

31. A method according to claim 1, wherein receiving light from the plurality of emitting surface areas of the sample comprises detecting light using an array of detectors, each detector in the array of detectors corresponding to a different one of the plurality of emitting surface areas.

35. A method according to claim 1, wherein determining composition information corresponding to the sub-surface region of the sample comprises determining composition information based on spectral information corresponding to expected components in the sub-surface region.

36. A method according to claim 1, further comprising determining spatial composition information corresponding to the sub-surface region of the sample.

37. A method according to claim 36, wherein determining spatial composition information comprises using position information associated with the plurality of emitting surface areas.

5-6. -6. (canceled)

8-11. -11. (canceled)

18. (canceled)

20-22. -22. (canceled)

27-30. -30. (canceled)

32-34. -34. (canceled)

38. An apparatus for measuring a composition of a sample, comprising:

an illumination system to illuminate an illumination surface area of the sample;

a plurality of light receivers to receive light from a plurality of emitting surface areas of the sample, each emitting surface area at a different location, the received light scattered by the sample;

wherein a cumulative area of the illumination surface area is greater than a cumulative area of two emitting surface areas of the plurality of emitting surface areas;

a spectrum analyzer optically coupled to receive light received by the plurality of light receivers, the spectrum analyzer configured to generate corresponding spectral content information associated with each emitting surface area of the sample; and

a computing device communicatively coupled to the spectrum analyzer, the computing device configured to determine composition information corresponding to a sub-surface region of the sample based on the generated spectral content information.

39. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area having a cumulative area substantially the same as a cumulative area of the plurality of surface areas.

40. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area having a cumulative area substantially less than a cumulative area of the plurality of surface areas.

41. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area having a cumulative area substantially greater than a cumulative area of the plurality of surface areas.

43. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area having a cumulative area greater than a cumulative area of four emitting surface areas of the plurality of emitting surface areas.

47. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area having a cumulative area greater than a cumulative area of eight emitting surface areas of the plurality of emitting surface areas.

50. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area as one contiguous area.

51. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area as a plurality of separate areas.

52. An apparatus according to claim 38, wherein illumination system comprises a spot shaper to shape the illumination surface area.

53. An apparatus according to claim 52, wherein the illumination system is configured to generate a shape of the illumination surface area including at least one of a circle, an oval, a square, a rectangle, a triangle, a line, an I shape, or a cross.

54. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area to overlap at least partially with at least some of the plurality of surface areas.

56. An apparatus according to claim 38, wherein the illumination system is configured to generate the illumination surface area to not overlap with the plurality of surface areas.

61. An apparatus according to claim 38, wherein the plurality of light receivers comprises a plurality of optical fibers, each optical fiber corresponding to a different one of the plurality of emitting surface areas.

62. An apparatus according to claim 61, further comprising a probe having the plurality of optical fibers arranged in at least one of a circle, an oval, at least one semi-circle, at least one V shape, and at least one line.

63. An apparatus according to claim 62, wherein the optical fibers from the plurality of optical fibers are included within an interior of the circle.

64. An apparatus according to claim 62, wherein the optical fibers from the plurality of optical fibers are only arranged on a circumference of the circle.

69. An apparatus according to claim 38, wherein the plurality of light receivers comprises an array of detectors, each detector in the array of detectors corresponding to a different one of the plurality of emitting surface areas.

73. An apparatus according to claim 38, wherein the computing device is configured to determine composition information based on spectral information corresponding to expected components in the sub-surface region.

74. An apparatus according to claim 38, wherein the computing device is further configured to determine spatial composition information corresponding to the sub-surface region of the sample.

75. An apparatus according to claim 74, wherein the computing device is further configured to determine spatial composition information using position information associated with the plurality of emitting surface areas.

42. (canceled)

44-46. -46. (canceled)

48-49. -49. (canceled)

55. (canceled)

57-60. -60. (canceled)

65-68. -68. (canceled)

70-72. -72. (canceled)