Enzymatic Biosensors With Enhanced Activity Retention For Detection Of Organic Compounds
First Claim
1. A distal tip of a biosensor transducer for use in detecting an organic compound susceptible to deamination, the tip comprising:
- a biocomponent comprising one or more enzymes for carrying out deamination of the organic compound, said biocomponent immobilized to a surface of the tip by one or more of (a) entrapment within a hydrogel;
(b) entrapment within a polymeric network;
(c) encapsulation;
(d) covalent bonding; and
(e) adsorption;
wherein said biocomponent has been treated to maintain a period of enzymatic efficacy; and
wherein said biocomponent is stabilized to said tip by one or more of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip.
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Accused Products
Abstract
Enzymatic biosensors and methods of producing distal tips for biosensor transducers for use in detecting one or more analytes selected from organic compounds susceptible to dehalogenation, organic compounds susceptible to oxygenation, organic compounds susceptible to deamination, organosulfate compounds susceptible to hydrolysis, and organophosphate compounds susceptible to hydrolysis are disclosed herein, as well as biosensor arrays, methods of detecting and quantifying analytes within a mixture, and devices and methods for delivering reagents to enzymes disposed within the distal tip of a biosensor.
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Citations
20 Claims
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1. A distal tip of a biosensor transducer for use in detecting an organic compound susceptible to deamination, the tip comprising:
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a biocomponent comprising one or more enzymes for carrying out deamination of the organic compound, said biocomponent immobilized to a surface of the tip by one or more of (a) entrapment within a hydrogel;
(b) entrapment within a polymeric network;
(c) encapsulation;
(d) covalent bonding; and
(e) adsorption;wherein said biocomponent has been treated to maintain a period of enzymatic efficacy; and wherein said biocomponent is stabilized to said tip by one or more of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A distal tip of a biosensor transducer for use in detecting an organic compound susceptible to deamination, the tip comprising:
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a biocomponent comprising at least one enzyme selected from the group consisting of hydrolases from EC family 3.5 and lyases from EC family 4.3 for carrying out deamination of the organic compound, said biocomponent immobilized to a surface of the tip by one or more of (a) entrapment within a hydrogel;
(b) entrapment within a polymeric network;
(c) encapsulation;
(d) covalent-bonding; and
(e) adsorption,wherein said biocomponent is stabilized to said tip by one or more of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip. - View Dependent Claims (9, 10, 11, 12, 13)
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14. A distal tip of a biosensor transducer for use in detecting organic compounds, the tip comprising:
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a biocomponent comprising at least one enzyme selected from the group consisting of hydrolases from EC family 3.5 and lyases from EC family 4.3 for carrying out deamination of one organic compound; and one or more of (i) an oxygenase from EC family 1.13 for carrying out an oxidation of one organic compound, (ii) an oxygenase from EC family 1.14 for carrying out an oxidation of one organic compound, (iii) a dehalogenase for carrying out dehalogenation of one organic compound, and (iv) a hydrolase from subclass EC 3.1 for carrying out hydrolysis of one organic compound, said biocomponent immobilized to a surface of the tip by one or more of (a) entrapment within a hydrogel;
(b) entrapment within a polymeric network;
(c) encapsulation;
(d) covalent-bonding; and
(e) adsorption,wherein said biocomponent is stabilized to said tip by one or more of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification