Materials and Procedures for the Purification of Cells

• US 20090221074A1
• Filed: 04/24/2009
• Published: 09/03/2009
• Est. Priority Date: 11/08/1996
• Status: Active Grant

• Current Assignee: Morphogenesis, Inc. (RadioIO, Inc.)
• Original Assignee: Morphogenesis, Inc. (RadioIO, Inc.)
• Inventors: Lawman, Michael J.P., Lawman, Patricia
• Granted Patent: US 8,030,069 B2
• Time in Patent Office: Days
• US Class Current: 435/378
• CPC Class Codes: C07K 16/2896 against molecules with a "CD"-designation, not provided for elsewhere
• CPC Class Codes: C07K 17/08 the carrier being a synthetic polymer
• CPC Class Codes: C12N 5/0087 Purging against subsets of blood cells, e.g. purging alloreactive T cells
• CPC Class Codes: C12N 5/0647 Haematopoietic stem cells; Uncommitted or multipotent progenitors
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

The subject invention provides new materials and methods for the efficient isolation and purification of stem cells. Specifically, conductive immunopolymers with stem cell specific antibodies can be used to remove stem cells from biological fluids.

Claims

1. A conductive polymer matrix comprising a molecule having binding specificity for a target molecule.

2. The conductive polymer matrix according to claim 1, wherein said molecule having binding specificity for a target molecule is an antibody, or an antigen binding fragment thereof.

3. The conductive polymer matrix according to claim 2, wherein said antibody is a monoclonal antibody.

4. The conductive polymer matrix according to claim 2, wherein said antibody binds to a CD34 determinant.

5. The conductive polymer matrix according to claim 1, wherein said molecule having binding specificity for a target molecule is selected from the group consisting of Fc receptor, Protein G, and avidin or avidin-related molecules.

6. A method for isolating a target cell from a sample comprising a mixture of cells, said method comprising contacting said mixture of cells with a conductive polymer matrix, wherein said conductive polymer matrix comprises a molecule having binding specificity for a target molecule expressed on said target cell;

binding of said target cell to said molecule having binding specificity for said target cell; and

releasing said molecule having binding specificity for said target molecule from said conductive polymer matrix.

7. The method according to claim 6, wherein said method further comprises washing said polymer matrix to remove unbound material.

8. The method according to claim 6, wherein said conductive polymer matrix comprises an antibody.

9. The method according to claim 8, wherein said antibody is a monoclonal antibody.

10. The method according to claim 8, wherein said antibody binds to a CD34 determinant.

11. The method according to claim 6, wherein said target cell is a stem cell.

12. The method according to claim 6, wherein said conductive polymer matrix is formed using enzyme generated means.

13. A device for isolating a target cell from a mixture of cells, said device comprising a conductive polymer matrix comprising a molecule having binding specificity for a target molecule.

14. The device according to claim 13, wherein said molecule having binding specificity for a target molecule is an antibody, or an antigen binding fragment thereof.

15. The device according to claim 14, wherein said antibody is a monoclonal antibody.

16. The device according to claim 14, wherein said antibody binds to a CD34 determinant.

17. The device according to claim 13, wherein said molecule having binding specificity for a target molecule is selected from the group consisting of Fc receptor, Protein G, and avidin or avidin-related molecules.