SMALL MOLECULE PRINTING
First Claim
1. An array comprising:
- a plurality of more than one type of chemical compound attached to a solid support through an isocyanate-derived linker or a isothiocyanate-derived linker, wherein the density of said array of compounds comprises at least 100 spots per cm2.
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Accused Products
Abstract
The present invention provides compositions and methods to facilitate the identification of compounds that are capable of interacting with a biological macromolecule of interest. In one aspect, a composition is provided that comprises an array of one or more types of chemical compounds attached to a solid support using isocyanate or isothiocyanate chemistry, wherein the density of the array of compounds is at least 1000 spots per cm2. In general, these inventive arrays are generated by: (1) providing a solid support, wherein said solid support is functionalized with an isocyanate or isothiocyanate moiety capable of interacting with a desired chemical compound to form a covalent attachment; (2) providing one or more solutions of one or more types of compounds to be attached to the solid support; (3) delivering said one or more types of compounds to the solid support; and (4) catalyzing the attachment of the compound to the solid support, whereby an array is formed and the array of compounds has a density of at least 1000 spots per cm2. In another aspect, the present invention provides methods for utilizing these arrays to identify small molecule partners for biological macromolecules of interest.
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Citations
51 Claims
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1. An array comprising:
a plurality of more than one type of chemical compound attached to a solid support through an isocyanate-derived linker or a isothiocyanate-derived linker, wherein the density of said array of compounds comprises at least 100 spots per cm2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 43, 44, 45, 46, 47)
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35. An array comprising:
a plurality of one or more types of chemical compounds attached to a solid support through an isocyanate-derived linker or an isothiocyanate-derived linker, wherein the density of said array of compounds comprises at least 100 spots per cm2.
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36. A method for forming an array of chemical compounds, the method comprising steps of:
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providing a solid support, wherein said solid support is functionalized with a weakly electrophilic moiety capable of interacting with more than one type of chemical compound to form a covalent linkage; providing one or more solutions of more than one type of chemical compounds to be attached to the solid support; delivering said one or more solutions of said more than one type of chemical compounds to the solid support, whereby each of said chemical compounds is attached to the solid support through a covalent interaction, and whereby said array of compounds has a density of at least 100 spots per cm2; and exposing the solid support to a base in vapor form under conditions sufficient to catalyze covalent attachment of the compounds to the support. - View Dependent Claims (37, 38, 39, 40, 41, 42)
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- 48. A solid support comprising a solid support derivatized with isocyanate moieties.
- 50. A solid support comprising a solid support derivatized with isothiocyanate moieties.
Specification