Aptamers that bind thrombin with high affinity
First Claim
1. An aptamer that binds to a thrombin target, wherein the aptamer decreases or inhibits thrombin mediated coagulation and the aptamer is ARC2172 (SEQ ID NO 294) or an aptamer that has substantially the same ability as ARC2172 (SEQ ID NO 294) to decrease or inhibit thrombin mediated coagulation, wherein the aptamer binds to human thrombin with a KD of less than 1 nM and wherein the aptamer is 55 nucleotides or less in length.
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Accused Products
Abstract
The invention provides aptamers capable of binding to thrombin useful as therapeutics for and diagnostics of coagulation related disorders and/or other diseases or disorders in which thrombin has been implicated. The invention further provides materials and methods for the administration of aptamers capable of binding to thrombin.
9 Citations
39 Claims
- 1. An aptamer that binds to a thrombin target, wherein the aptamer decreases or inhibits thrombin mediated coagulation and the aptamer is ARC2172 (SEQ ID NO 294) or an aptamer that has substantially the same ability as ARC2172 (SEQ ID NO 294) to decrease or inhibit thrombin mediated coagulation, wherein the aptamer binds to human thrombin with a KD of less than 1 nM and wherein the aptamer is 55 nucleotides or less in length.
-
6. An aptamer that binds to thrombin selected from the group consisting of:
- SEQ ID NOs 9-41, 43-191, 193-204, 208-304, 307-329, 331-332, 334, 336-337, 340-392, 396-397, 400, and 402-440.
-
7. An aptamer that binds to thrombin comprising the following nucleic acid sequence:
- CCTAGGTTGGGTAGGGTGGTGG (SEQ ID NO;
441). - View Dependent Claims (8, 14, 15, 16)
- CCTAGGTTGGGTAGGGTGGTGG (SEQ ID NO;
-
9. An aptamer comprising the following nucleic acid sequence N1N2N3TAGGTTGGGTAGGGTGGTN′
-
3N′
2N′
1 (SEQ ID NO;
442) wherein N1, N2, or N3 is any nucleotide that forms a base pair with N′
1, N′
2 or N′
3 respectively, wherein N1, N2, and N3, may each be the same nucleotide or different nucleotides and the aptamer decreases or inhibits thrombin mediated coagulation. - View Dependent Claims (10, 11, 12, 13)
-
3N′
-
17. (canceled)
Specification