Grating waveguide structure for reinforcing an excitation field and use thereof

• US 20090224173A1
• Filed: 12/31/2008
• Published: 09/10/2009
• Est. Priority Date: 04/14/2000
• Status: Abandoned Application

• Current Assignee: Gerd Marowsky, Gert Ludwig Duveneck, Markus Ehrat, Martin Andreas Bopp, Michael Pawlak
• Original Assignee: Gerd Marowsky, Gert Ludwig Duveneck, Markus Ehrat, Martin Andreas Bopp, Michael Pawlak
• Inventors: Marowsky, Gerd, Pawlak, Michael, Bopp, Martin Andreas, Duveneck, Gert Ludwig, Ehrat, Markus
• Application Number:
  US12/318,568
• Publication Number:
  US 20090224173A1
• Time in Patent Office: Days
• US Class Current: 250/459.100
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/774 the reagent being on a grating or periodic structure
• CPC Class Codes: G02B 6/124 Geodesic lenses or integrated gratings

Abstract

The invention relates to a variable embodiment of a grating waveguide structure, based on a planar thin-film waveguide with a first optically transparent layer (a) on a second optically transparent layer (b) having a lower refractive index than layer (a), and a grating structure (c) modulated in layer (a), wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) is enhanced by at least a factor of 100 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light. The invention also relates to an optical system with an excitation light source and an embodiment of a grating waveguide structure according to the invention, and to a method for enhancing an excitation light intensity, and to the use thereof in bioanalytical detection processes, in non-linear optics or in telecommunications or communications industry.

Claims

1-60. -60. (canceled)

61. A method for amplification of an excitation light intensity, using a grating wave guide structure comprising a layer (a), transparent at least at one excitation wavelength, on a second layer (b) with lower refractive index than layer (a), also transparent at least at said excitation wavelength, and at least one grating structure (c) modulated in layer (a), wherein:

a. the refractive index of the first optically transparent layer (a) is larger than 1.8,

b. grating structures (c) modulated in layer (a) have a period of 200 nm-1000 nm and a modulation depth of 3 nm to 100 nm,

c. layer thickness of the optically transparent layer (a) allows guiding of one to three modes at a given wavelength

wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) on a grating structure (c) modulated in layer (a) of a grating waveguide structure, is enhanced by at least a factor of 100 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light and wherein luminescence label or biomolecules capable of luminescence are excited by two-photon absorption.

62. The method according to claim 61, wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) on a grating structure (c) modulated in layer (a) is enhanced by at least a factor of 1 000 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light.

63. The method according to claim 61, wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) on a grating structure (c) modulated in layer (a) is enhanced by at least a factor of 10 000 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light.

64. The method according to claim 61, wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) on a grating structure (c) modulated in layer (a) is enhanced by at least a factor of 100 000 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light.

65. The method according to claim 61, wherein the excitation light intensity on layer (a) is sufficiently large to excite luminescence from a molecule located on the surface of layer (a) or at a distance below 200·

nm from layer (a) by two-photon absorption.

66. The method according to claim 65, wherein the excitation light intensity on layer (a) is sufficiently large simultaneously on an area of at least 1 mm² on said grating waveguide structure to excite luminescence from molecules located on the surface of layer (a) or at a distance below 200·

nm from layer (a) by two-photon absorption.

67. The method according to claim 61, wherein a luminescence generated on or in the near-field of layer (a) by two-photon absorption is transmitted to an adjacent grating waveguide structure upon outcoupling by a grating structure (c).

68. The method according to claim 61, wherein the grating waveguide structure comprises continuous, unmodulated regions of layer (a), which are preferably arranged in direction of propagation of an excitation light incoupled by a grating structure (c) and guided in layer (a).

69. The method according to claim 61, wherein the grating waveguide structure comprises a multitude of grating structures (c) with identical or different period, optionally adjacent thereto with continuous, unmodulated regions of layer (a) on a common, continuous substrate.

70. The method according to claim 61, wherein a luminescence generated on or in the near-field of layer (a) by two-photon absorption, is coupled at least partially into layer (a) and is propagated to adjacent regions of said grating waveguide structure by guiding in layer (a).

71. The method according to claim 61, wherein the intensity of the excitation light on layer (a) and within layer (a) is sufficiently high, at least in the region of the grating structure (c), for switching the transmission properties of the grating structure (c) for a light signal guided in layer (a).

72. The method according to claim 71, characterized in that it allows for switching the transmission properties of the grating structure (c) by means of an excitation light launched from the outside of layer (a) onto said grating structure.

73. The method according to claim 71, wherein said grating structure (c) is provided as a “

Bragg grating”

, and the switching function is based on the change of the grating function from transmission to reflection of a light signal guided in layer (a), due to a change of the optical refractive index in the region of the grating structure caused by the amplified excitation light intensity in layer (a).

74. The method according to claim 71, wherein a first excitation light as a signal light, in the form of temporal pulse or continuously, is coupled into layer (a) by a first grating structure and is guided in layer (a), until said incoupled, guided signal light arrives in the region of another grating structure (c′

) structured in layer (a), with the same or a grating period different from the one of said first grating structure (c), an excitation light irradiated from externally, as a switching light in the form of a temoral pulse or continuously, being incoupled into layer (a) by means of said second grating structure, and, due to the associated amplification of this switching light by at least a factor of 100 on layer (a) and within layer (a) at least in the region of the grating structure, in comparison with the intensity of this excitation light on a substrate surface without incoupling of the excitation light, the refractive index of layer (a) is changed at least in the region of grating structure (c′

), due to high third-order nonlinearity, so that the function of said grating structure (c′

) is changed from transmission to reflection of said signal light.

81. The method according to claim 61, wherein (1) the isotropically emitted luminescence or (2) the luminescence that is coupled back into the optically transparent layer (a) and outcoupled by grating structures (c) or luminescences of both parts (1) and (2) simultaneously are measured.

82. The method according to claim 61, wherein, for the generation of luminescence, a luminescence dye or luminescent nanoparticle is used as a luminescence label, which can be excited at a wavelength between 200 nm and 1100 nm.

83. The method according to claim 82, wherein said luminescence label is excited by two-photon absorption.

84. The method according to claim 83, wherein said luminescence label is excited to an ultraviolet or blue luminescence by two-photon absorption of an excitation light in the visible or near infrared.

85. The method according to claim 82, wherein the luminescence label is bound to the analyte or, in a competitive assay, to an analyte analogue or, in a multi-step assay, to one of the binding partners of the immobilized biological or biochemical or synthetic recognition elements or to the biological or biochemical or synthetic recognition elements.

86. The method according to claim 82, wherein a second or more luminescence labels of similar or different excitation wavelength as the first luminescence label and similar or different emission wavelength are used.

87. The method according to claim 86, wherein the second or more luminescence labels can be excited at the same wavelength as the first luminescence dye, but emit at other wavelengths.

88. The method according to claim 86, wherein the excitation and emission spectra of the applied luminescent dyes do not or only partially overlap.

89. The method according to claim 86, wherein charge or optical energy transfer from a first luminescent dye acting as a donor to a second luminescent dye acting as an acceptor is used for the detection of the analyte.

90. The method according to claim 61, wherein the one or more luminescences and/or determinations of light signals at the excitation wavelengths are performed polarization-selective, wherein preferably the one or more luminescences are measured at a polarization that is different from the one of the excitation light.

91. The method according to claim 61, wherein molecules located on the surface of layer (a) or at distance of less than 200 nm from layer (a) are trapped within this distance, due to the large amplification of an irradiated excitation light on layer (a) and within layer (a), as the high surface-confined excitation light intensity and its increasing gradient in direction towards the surface exposes these molecules to the effect of an “

optical tweezers”

.

92. The method according to claim 61 for the simultaneous or sequential, quantitative or qualitative determination of one or more analytes of the group comprising antibodies or antigens, receptors or ligands, chelators or “

histidin-tag components”

, oligonucleotides, DNA or RNA strands, DNA or RNA analogues, enzymes, enzyme cofactors or inhibitors, lectins and carbohydrates.

93. The method according to claim 61, wherein the samples to be examined are naturally occurring body fluids, such as blood, serum, plasma, lymph or urine or egg yolk, or optically turbid liquids or surface water or soil or plant extracts or bio- or process broths or are taken from biological tissue pieces.

94. The method according to claim 61 for the determination of chemical, biochemical or biological analytes in screening methods in pharmaceutical research, combinatorial chemistry, clinical and preclinical development, for real-time binding studies and the determination of kinetic parameters in affinity screening and in research, for qualitative and quantitative analyte determinations, especially for DNA- and RNA analytics, for the generation of toxicity studies and the determination of expression profiles and for the determination of antibodies, antigens, pathogens or bacteria in pharmaceutical product development and research, human and veterinary diagnostics, agrochemical product development and research, for patient stratification in pharmaceutical product development and for the therapeutic drug selection, for the determination of pathogens, nocuous agents and germs, especially of salmonella, prions and bacteria, in food and environmental analytics.

95. The method according to claim 61 in nonlinear optics or telecommunication or communication techniques.

96. The method according to claim 61 for surface-confined investigations which require the application of very high excitation light intensities and/or excitation durations, such as studies of photostabilities of materials, photocatalytic processes etc.

75. A method for the detection of one or more analytes by luminescence detection, in one or more samples on one or more measurement areas of a grating waveguide structure comprising a layer (a), transparent at least at one excitation wavelength, on a second layer (b) with lower refractive index than layer (a), also transparent at least at said excitation wavelength and at least one grating structure (c) modulated in layer (a), wherein:

d. the refractive index of the first optically transparent layer (a) is larger than 1.8,

e. grating structures (c) modulated in layer (a) have a period of 200 nm-1000 nm and a modulation depth of 3 nm to 100 nm,

f. layer thickness of the optically transparent layer (a) allows guiding of one to three modes at a given wavelength

for the determination of one or more luminescences from a measurement area or from an array of at least two or more laterally separated measurement areas (d) or of at least two or more laterally separated segments comprising several measurement areas on said grating waveguide structure, wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) is enhanced by at least a factor of 100 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light and wherein luminescence label or biomolecules capable of luminescence are excited by two-photon absorption.

76. The method according to claim 75, wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) is enhanced by at least a factor of 1 000 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light.

77. The method according to claim 75, wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) is enhanced by at least a factor of 10,000 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light.

78. The method according to claim 75, wherein the intensity of an excitation light irradiated at the resonance angle for incoupling into layer (a) is enhanced by at least a factor of 100,000 on layer (a) and within layer (a), at least in the region of the grating structure (c), in comparison with the intensity of said excitation light on a substrate surface without incoupling of the excitation light.

79. The method according to claim 75, wherein the excitation light intensity on layer (a) is sufficiently large to excite luminescence from a molecule located on the surface of layer (a) or at a distance below 200·

nm from layer (a) by two-photon absorption.

80. The method according to claim 79, wherein the excitation light intensity on layer (a) is sufficiently large simultaneously on an area of at least 1 mm² on said grating waveguide structure to excite luminescence from molecules located on the surface of layer (a) or at a distance below 200·

nm from layer (a) by two-photon absorption.

97. The method according to claim 75, wherein (1) the isotropically emitted luminescence or (2) the luminescence that is coupled back into the optically transparent layer (a) and outcoupled by grating structures (c) or luminescences of both parts (1) and (2) simultaneously are measured.

98. The method according to claim 75, wherein, for the generation of luminescence, a luminescence dye or luminescent nanoparticle is used as a luminescence label, which can be excited at a wavelength between 200 nm and 1100 nm.

99. The method according to claim 98, wherein said luminescence label is excited by two-photon absorption.

100. The method according to claim 99, wherein said luminescence label is excited to an ultraviolet or blue luminescence by two-photon absorption of an excitation light in the visible or near infrared.

101. The method according to claim 98, wherein the luminescence label is bound to the analyte or, in a competitive assay, to an analyte analogue or, in a multi-step assay, to one of the binding partners of the immobilized biological or biochemical or synthetic recognition elements or to the biological or biochemical or synthetic recognition elements.

102. The method according to claim 98, wherein a second or more luminescence labels of similar or different excitation wavelength as the first luminescence label and similar or different emission wavelength are used.

103. The method according to claim 102, wherein the second or more luminescence labels can be excited at the same wavelength as the first luminescence dye, but emit at other wavelengths.

104. The method according to claim 102, wherein the excitation and emission spectra of the applied luminescent dyes do not or only partially overlap.

105. The method according to claim 102, wherein charge or optical energy transfer from a first luminescent dye acting as a donor to a second luminescent dye acting as an acceptor is used for the detection of the analyte.

106. The method according to claim 75, wherein the one or more luminescences and/or determinations of light signals at the excitation wavelengths are performed polarization-selective, wherein preferably the one or more luminescences are measured at a polarization that is different from the one of the excitation light.

107. The method according to claim 75, wherein molecules located on the surface of layer (a) or at distance of less than 200 nm from layer (a) are trapped within this distance, due to the large amplification of an irradiated excitation light on layer (a) and within layer (a), as the high surface-confined excitation light intensity and its increasing gradient in direction towards the surface exposes these molecules to the effect of an “

optical tweezers”

.

108. The method according to claim 75 for the simultaneous or sequential, quantitative or qualitative determination of one or more analytes of the group comprising antibodies or antigens, receptors or ligands, chelators or “

histidin-tag components”

, oligonucleotides, DNA or RNA strands, DNA or RNA analogues, enzymes, enzyme cofactors or inhibitors, lectins and carbohydrates.

109. The method according to claim 75, wherein the samples to be examined are naturally occurring body fluids, such as blood, serum, plasma, lymph or urine or egg yolk, or optically turbid liquids or surface water or soil or plant extracts or bio- or process broths or are taken from biological tissue pieces.

110. The method according to claim 75 for the determination of chemical, biochemical or biological analytes in screening methods in pharmaceutical research, combinatorial chemistry, clinical and preclinical development, for real-time binding studies and the determination of kinetic parameters in affinity screening and in research, for qualitative and quantitative analyte determinations, especially for DNA- and RNA analytics, for the generation of toxicity studies and the determination of expression profiles and for the determination of antibodies, antigens, pathogens or bacteria in pharmaceutical product development and research, human and veterinary diagnostics, agrochemical product development and research, for patient stratification in pharmaceutical product development and for the therapeutic drug selection, for the determination of pathogens, nocuous agents and germs, especially of salmonella, prions and bacteria, in food and environmental analytics.

111. The method according to claim 75 in nonlinear optics or telecommunication or communication techniques.

112. The method according to claim 75 for surface-confined investigations which require the application of very high excitation light intensities and/or excitation durations, such as studies of photostabilities of materials, photocatalytic processes etc.