ENCODED SOLID SUPPORTS FOR BIOLOGICAL PROCESSING AND ASSAYS USING SAME
6 Assignments
0 Petitions
Accused Products
Abstract
Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The data storage units are non-volatile antifuse memories or volatile memories, such as EEPROMS, DRAMS or flash memory. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for electronically tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.
97 Citations
200 Claims
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1-163. -163. (canceled)
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164. A multiplexed assay, comprising:
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attaching each molecule of a plurality of molecules to one of a plurality of support matrices, each support matrix adapted for attachment and/or synthesis of molecules, wherein the support matrix is physically linked to a memory device, the memory device comprising an electromagnetically-readable memory containing encoded data to uniquely identify a molecule attached to the support matrix;
exposing the plurality of molecules to one or more processing conditions;
placing the plurality of support matrices with the attached molecules into a common vessel; and
measuring biochemical processes within the common vessel;
wherein each of the plurality of support matrices has different encoded data in its linked memory device so that when the plurality of-support matrices is placed into the common vessel, the molecule associated with each support matrix has a unique identity associated therewith. - View Dependent Claims (167, 168, 171, 172, 181, 182, 183, 185, 186, 187, 188, 189, 190, 191)
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165. (canceled)
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166. (canceled)
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169-170. -170. (canceled)
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173. An assay for evaluating a plurality of molecules, comprising:
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linking each of the molecules to a separate support matrix with an electromagnetically-readable memory containing encoded data with a unique identity, wherein the unique identity is associated with the molecule linked to the support matrix. exposing the plurality of molecules and their corresponding support matrices to one or more reagents;
placing a plurality of support matrices with the linked molecules into a common vessel;
measuring biochemical processes within the common vessel using analytical instrumentation; and
generating a user readable display of measurements made by the analytical instrumentation. - View Dependent Claims (175, 176, 178, 179, 180, 184, 192, 193, 194, 195, 196, 197)
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174. (canceled)
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177. (canceled)
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198. A multiplexed assay, comprising:
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attaching each molecule of a plurality of molecules to a support particle, each support particle adapted for attachment and/or synthesis of molecules, wherein the support particle comprises a unique optically-encoded identifier for uniquely identifying a molecule attached to the support particle;
exposing the plurality of support particles with the attached molecules to one or more processing conditions to produce a plurality of analytes;
placing the plurality of support particles with the attached molecules into a multi-well plate;
measuring the plurality of analytes within the multi-well plate; and
before, during or after the step of measuring, reading the encoded data with an optical reader to determine the unique identity of the support particle;
wherein each of the plurality of support particles has different encoded data in its linked memory device so that when the plurality of support particles is placed into the multi-well plate, the molecule associated with each support particle has a unique identity associated therewith. - View Dependent Claims (199, 200)
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Specification