Method of detecting vibrio parahaemolyticus via real-time PCR-hybridization
First Claim
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1. A method of detecting Vibrio parahaemolyticus, comprising the steps of:
- obtaining a sample,preparing sample for inclusion into assay,running real-time PCR, anddetecting toxR gene and tdh2 gene,wherein running real-time PCR uses an assay having two pairs of oligonucleotide primers and two pairs of probes.
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Abstract
The present invention relates to an assay and methods for determining the presence of pathogenic V. parahaemolyticus in a sample. Determination should be made by detector. Via the assay, rapid, real-time detection of V. parahaemolyticus is possible.
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10 Claims
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1. A method of detecting Vibrio parahaemolyticus, comprising the steps of:
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obtaining a sample, preparing sample for inclusion into assay, running real-time PCR, and detecting toxR gene and tdh2 gene, wherein running real-time PCR uses an assay having two pairs of oligonucleotide primers and two pairs of probes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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- 9. An assay for rapid detection of Vibrio parahaemolyticus comprising two pairs of oligonucleotide primers selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 5, and SEQ ID NO. 6, and two probes consisting of SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 7, and SEQ ID NO. 8.
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