Methods for pathogen detection

• US 20090227005A1
• Filed: 09/28/2007
• Published: 09/10/2009
• Est. Priority Date: 03/27/2007
• Status: Abandoned Application

• Current Assignee: Gearbox LLC (Intellectual Ventures LLC)
• Original Assignee: Searete LLC (Intellectual Ventures LLC)
• Inventors: Rinaldo, John D. JR., Lord, Robert W., Jung, Edward K.Y., Levien, Royce A., Wood, Lowell L. JR., Malamud, Mark A., Leuthardt, Eric C.
• Application Number:
  US11/906,124
• Publication Number:
  US 20090227005A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 35/00 Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Abstract

The present disclosure relates to methods, systems, devices, and microfluidic chips that may be used for the detection of pathogens.

Claims

1-78. -78. (canceled)

79. A system comprising:

circuitry for combining one or more samples with one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes; and

circuitry for separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples.

80. The system of claim 79, wherein the circuitry for combining one or more samples with one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes comprises:

circuitry for combining the one or more samples with at least one magnetically active antibody, aptamer, polynucleotide, or polypeptide.

81. The system of claim 79, wherein the circuitry for separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

circuitry for separating the one or more magnetically active pathogen indicator complexes through use of magnetic attraction or magnetic repulsion.

82. The system of claim 79, wherein the circuitry for separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

circuitry for separating the one or more magnetically active pathogen indicator complexes through use of one or more ferrofluids.

83. The system of claim 79, further comprising:

circuitry for analyzing the one or more samples.

85. The system of claim 83, further comprising:

circuitry for identifying one or more pathogens present within the one or more samples.

87. The system of claim 85, wherein the circuitry for identifying one or more pathogens present within the one or more samples comprises:

circuitry for displaying an identity of the one or more pathogens present within the one or more samples.

84. (canceled)

86. (canceled)

88. A system comprising:

circuitry for combining one or more samples with one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes; and

circuitry for separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially antiparallel flow with the one or more samples.

89. The system of claim 88, wherein the circuitry for combining one or more samples with one or more magnetically active pathogen indicator binding agents that can bind to one or more pathogen indicators associated with the one or more samples to form one or more magnetically active pathogen indicator complexes comprises:

circuitry for combining the one or more samples with at least one magnetically active antibody, aptamer, polynucleotide, or polypeptide.

90. The system of claim 88, wherein the circuitry for separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially antiparallel flow with the one or more samples comprises:

circuitry for separating the one or more magnetically active pathogen indicator complexes through use of magnetic attraction or magnetic repulsion.

91. The system of claim 88, wherein the circuitry for separating the one or more magnetically active pathogen indicator complexes from the one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially antiparallel flow with the one or more samples comprises:

circuitry for separating the one or more magnetically active pathogen indicator complexes through use of one or more ferrofluids.

92. The system of claim 88. further comprising:

circuitry for analyzing the one or more samples.

94. The system of claim 92, further comprising:

circuitry for identifying one or more pathogens present within the one or more samples.

96. The system of claim 94, wherein the circuitry for identifying one or more pathogens present within the one or more samples comprises:

circuitry for displaying an identity of the one or more pathogens present within the one or more samples.

93. (canceled)

95. (canceled)

97-122. -122. (canceled)

123. A system comprising:

circuitry for separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples.

124. The system of claim 123, wherein the circuitry for separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

circuitry for separating the one or more magnetically active pathogen indicator complexes through use of magnetic attraction or magnetic repulsion.

125. The system of claim 123, wherein the circuitry for separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially parallel flow with the one or more samples comprises:

circuitry for separating the one or more magnetically active pathogen indicator complexes through use of one or more ferrofluids.

126. The system of claim 123, further comprising:

circuitry for detecting one or more pathogen indicators.

128. The system of claim 126, further comprising:

circuitry for identifying one or more pathogens present within the one or more samples.

130. The system of claim 128, wherein the circuitry for identifying one or more pathogens present within the one or more samples comprises:

circuitry for displaying an identity of the one or more pathogens present within the one or more samples.

127. (canceled)

129. (canceled)

131. A system comprising:

circuitry for separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially antiparallel flow with the one or more samples.

133. The system of claim 131, wherein the circuitry for separating one or more magnetically active pathogen indicator complexes from one or more samples through use of one or more magnetic fields and one or more separation fluids that are in substantially antiparallel flow with the one or more samples comprises:

circuitry for separating the one or more magnetically active pathogen indicator complexes through use of one or more ferrofluids.

134. The system of claim 131, further comprising:

circuitry for detecting one or more pathogen indicators.

136. The system of claim 134, further comprising:

circuitry for identifying one or more pathogens present within the one or more samples.

138. The system of claim 136, wherein the circuitry for identifying one or more pathogens present within the one or more samples comprises:

circuitry for displaying an identity of the one or more pathogens present within the one or more samples.

135. (canceled)

137. (canceled)

139-192. -192. (canceled)