Apparatus for Performing Nucleic Acid Analysis

• US 20090227006A1
• Filed: 07/05/2007
• Published: 09/10/2009
• Est. Priority Date: 07/14/2006
• Status: Abandoned Application

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Roche Molecular Systems (Roche Holding AG)
• Inventors: Sarofim, Emad, Wahl, Hans-Peter, Hutter, Roland Christof, Elsenhans, Oliver, Kopp, Martin, Savatic, Goran
• Application Number:
  US12/373,276
• Publication Number:
  US 20090227006A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 2035/00346 Heating or cooling arrangements
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J

Abstract

The embodiments of the present invention are directed to an automatic apparatus (55), which is designed for performing nucleic acid analysis, optionally including sample preparation, with integrated disposable sample holding and processing devices (1), which devices (1) comprise a sample to be analyzed with the apparatus (55). The apparatus (55) comprises workstations (17, 40) for receiving, holding and processing such integrated disposable devices (1), a reagents station (56) for reagents (25), transfer means (36) for performing movements of an actuator, fluid actuation means for processing the sample (32) and reagents in said disposable devices (1), temperature control means (37) for heating or cooling, detection means for measuring a property of the processed samples (32), and control means for controlling the sample (32) preparation, the handling of the disposable devices (1) and the sample analysis performed by the apparatus (55).

Claims

1-59. -59. (canceled)

60. An automatic apparatus for performing nucleic acid sample analysis by using a disposable device for holding and processing samples with the apparatus, wherein the apparatus is designed and adapted for processing a disposable device and wherein said apparatus comprises:

at least one disposable device which is a fluidic device comprising a fluidic system designed for nucleic acid testing, which testing comprises the steps of capturing, amplification and detection within said disposable device, the disposable device also comprising a binding chamber containing a solid phase for solid phase extraction of a component of the sample to be analyzed, an amplification chamber and a fluid channel connecting the amplification chamber to the binding chamber, the disposable device further comprising at least one fluid interface for interacting with the fluid actuation means, said fluid interface providing fluid to the disposable in a vertical direction of flow when the disposable device is operated in the apparatus,

a detection station for receiving and holding at least one disposable device comprising fittings for guiding and holding the disposable devices, wherein said fittings are designed to enable vertical inserting and removing of the disposable device into and from the detection station by the transfer means,

at least one reagent station comprising at least one reagent to be used in the nucleic acid sample preparation and analysis process,

at least one transfer means for performing movements of an actuator of the apparatus within the apparatus,

at least one fluid actuation means for processing the sample and at least one reagent in said disposable devices, wherein the fluid actuation means comprises at least one of a means for transport and manipulation of fluid in the disposable,

at least one means for controlling the temperature by heating and cooling at least one of the disposable device and the sample,

detection means for measuring a property of the processed samples, said detection means comprising an optical detector for measuring an optical parameter of the liquid in the amplification and detection chamber through an optical measurement window which is provided in the amplification chamber of the disposable device, said disposable device being operated in the detection station, and the optical detector being arranged such that a measurement direction from the measurement window to the optical detector is horizontal, and

means for controlling the process of;

sample preparation, handling of the disposable devices and analysis of the sample performed by the apparatus.

61. The apparatus according to claim 60, wherein the means for controlling the temperature is adapted to provide heating and cooling of a liquid by a thermal conduction between the temperature control means and the amplification chamber, said liquid being present in the amplification chamber of said disposable device, said disposable device being operated in the detection station, wherein an effective direction of heat flow between the temperature control means and the amplification chamber is horizontal.

62. The apparatus according to claim 60, wherein the optical detector and the means for controlling the temperature are arranged on opposite sides of the amplification chamber and wherein the measurement direction from the measurement window to the optical detector is the same as the effective direction of heat flow from the temperature control means to the amplification chamber.

63. The apparatus according to claim 60, wherein the transfer means comprise an actuation head, said actuation head being automatically movable horizontally in one or two directions, whereby said transfer means is positioned above at least one element of the group consisting of:

the disposable devices, the sample and a reagent container, and wherein said actuation head is automatically movable in a vertical direction, with at least one means selected from the group consisting of;

means for providing pressure or vacuum, pneumatic, hydraulic pressure, temperature control, heat supply, heat removal, uptake and release of a sample and of a fluid being actuated.

64. The apparatus according to claim 60, wherein the device comprises at least one fluid interface to be connected to the fluid actuation means of the apparatus, the fluid interface being connected by a fluid channel to at least one chamber of the device, wherein the fluid interfaces are situated on at least one of the top- and bottom-surfaces of the body when the device is operated in the nucleic acid amplification apparatus.

65. The apparatus according to claim 60, wherein the apparatus comprises a sample preparation station for performing the step of lysis of the nucleic acid in a sample.

66. The apparatus according to claim 60, wherein the processing station is located at the same place in the apparatus as the sample preparation station such that the disposables do not have to be transported from the sample preparation station to the processing station but can be kept in place after preparation of the sample for performing the analysis.

67. The apparatus according to claim 60, wherein a lysis chamber is comprised within the disposable device, which lysis chamber is adapted for performing lysis of the nucleic acid in a sample.

68. The apparatus according to claim 60, wherein a sample transfer tip is provided which is adapted to receive a sample and to transfer said sample into said disposable device.

69. The apparatus according to claim 60, wherein the transfer means comprise a tip gripper, said tip gripper being adapted to automatically grip a sample tip adapted to place said sample tip into said disposable device being positioned in the detection station.

70. The apparatus according to claim 60, wherein the disposable device comprises at least one fluid inlet port adapted to provide fluidic communication between said fluidic system of the disposable device and an actuation means for supplying fluid into the fluidic system of said disposable device, and the apparatus comprises a corresponding fluid actuation means.

71. The apparatus according to claim 60, wherein the disposable device comprises at least one waste connector which is in fluidic communication with the fluidic system of the disposable device, and the apparatus comprises a corresponding fluid actuation means.

72. The apparatus according to claim 60, comprising at least one transfer means which comprises a gripping device for inserting the disposable device to be analyzed into a receptacle of the detection station and for removing the disposable device after processing and analysis.

73. The apparatus according to claim 60, wherein the apparatus provides to a disposable device placed in the apparatus in one element selected from a sample preparation station and a detection station, at least one of the means selected from the groups consisting of:

pressure, vacuum, pneumatic pressure, hydraulic pressure, temperature control, heat supply, heat removal, uptake and release of a sample or of a fluid.

74. The apparatus according to claim 60, wherein the fluid actuation means is adapted to provide fluid supply and fluid removal into or from the disposable device in a vertical direction of flow in said disposable device, the disposable device being operated in said apparatus, in a sample preparation station or the detection station, said fluid being a member selected from the group consisting of a sample, a reagent, and mixtures thereof.

75. The apparatus according to claim 60, wherein the device comprises at least one sealing point located close to a channel of the device, said channel leading from a chamber of the device to one element selected from the group consisting of:

an inlet port, an outlet port, a venting port of the device, and from a first chamber to a second chamber, wherein the channel located close to the sealing point can be sealed by a sealer of the apparatus in order to close said channel for interrupting a flow of liquid or gas through said channel, and the apparatus comprises a corresponding channel sealing equipment for closing the sealing point.

76. The apparatus according claim 60, wherein the apparatus comprises a fluid filling measuring means adapted for measuring the filling of the fluidic system of the disposable device, adapted for measuring the maximum filling of the amplification chamber, said fluid filling measuring means being positioned in the disposable detection station.

77. The apparatus according to claim 60, wherein the amplification chamber has an outlet and wherein an outlet channel is connected to said outlet, and wherein the fluid filling measuring means is arranged in said outlet channel of the amplification chamber close to the outlet of the amplification chamber being adapted to detect the maximum filling of the amplification chamber.

78. A system for performing nucleic acid sample analysis by using disposable devices for holding and processing a sample, said system comprising an automatic according to claim 60.

79. The system according to claim 78, wherein the detection station comprises fittings for guiding and holding the disposable devices, wherein said fittings are designed to enable vertical inserting and removing of the disposable device into and from the detection station by the transfer means.

80. The system according to claim 78, wherein the means for controlling the temperature is adapted to provide heating and cooling of a liquid by a thermal conduction between the means for controlling the temperature and the amplification chamber, said liquid being comprised in the amplification chamber of said disposable device, said disposable device being operated in the detection station, wherein an effective direction of heat flow between the temperature control means and the amplification chamber is horizontal.