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GLP-1 gene delivery for the treatment of type 2 diabetes

  • US 20090227660A1
  • Filed: 03/05/2008
  • Published: 09/10/2009
  • Est. Priority Date: 05/21/2002
  • Status: Abandoned Application
First Claim
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1. A method of normalizing blood glucose levels of a warm blooded animal having type 2 diabetes, comprising:

  • providing a transfection formulation comprising a cationic polymeric gene carrier complexed with a selected plasmid consisting essentially of;

    an expression facilitating sequence derived from chicken β

    -actin promoter and enhancer;

    an expression sequence comprising ATG (start codon) followed by a sequence SEQ ID NO;

    5 (CGTCAACGTCGT) coding for a furin cleavage site (FCS) and a sequence coding for the active form of GLP-1 (7-37) or derivatives thereof that are operably linked to said expression facilitating sequence in a proper charge ratio (positive charge of the copolymer/negative charge of the nucleic acid) that is optimally effective for both in vivo and in vitro transfection; and

    administering to the animal to be treated an effective amount of the composition such that the cell internalizes the GLP-1 gene.

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