RAPID DETECTION OF VOLATILE ORGANIC COMPOUNDS FOR IDENTIFICATION OF BACTERIA IN A SAMPLE

• US 20090230300A1
• Filed: 10/17/2008
• Published: 09/17/2009
• Est. Priority Date: 10/19/2007
• Status: Abandoned Application

• Current Assignee: BETH Israel Deaconess Medical Center (Caregroup Incorporated), Charles Stark Draper Laboratory Incorporated
• Original Assignee: BETH Israel Deaconess Medical Center (Caregroup Incorporated)
• Inventors: Kirby, James, Trevejo, Jose Miguel, Hoenigman, Shirley
• Application Number:
  US12/253,704
• Publication Number:
  US 20090230300A1
• Time in Patent Office: Days
• US Class Current: 250/282
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 2333/35 from Mycobacteriaceae (F)
• CPC Class Codes: G01N 2800/12 Pulmonary diseases
• CPC Class Codes: G01N 2800/52 Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Abstract

In various embodiments, the invention relates to a method for identifying the presence of particular bacteria in a sample. The method includes collecting a sample that includes or has been exposed to the particular bacteria and detecting, in the sample, at least one volatile organic compound indicative of the presence of the bacteria.

Claims

1. A method for identifying Staphylococcus aureus bacteria in a sample, the method comprising:

a. collecting a sample suspected of comprising Staphylococcus aureus bacteria; and

b. detecting one or more volatile organic compounds indicative of a presence of or response to treatment or resistance of the Staphylococcus aureus bacteria in the sample, the one or more volatile organic compounds being selected from the group consisting of methanethiol, dimethyl sulfide, 2,3-butanedione, 3-hydroxy-2-butanone, butyl acetate, and benzeneacetaldehyde.

2. The method of claim 1, wherein the one or more volatile organic compounds comprises methanethiol.

3. The method of claim 1, wherein the one or more volatile organic compounds comprises dimethyl sulfide.

4. The method of claim 1, wherein the one or more volatile organic compounds comprises 2,3-butanedione.

5. The method of claim 1, wherein the one or more volatile organic compounds comprises 3-hydroxy-2-butanone.

6. The method of claim 1, wherein the one or more volatile organic compounds comprises butyl acetate.

7. The method of claim 1, wherein the one or more volatile organic compounds comprises benzeneacetaldehyde.

8. The method of claim 1, wherein a combination of two or more volatile organic compounds is indicative of the presence of or response to treatment or resistance of Staphylococcus aureus in the sample.

9. The method of claim 1, wherein the sample was exposed to a candidate therapy for treating Staphylococcus aureus.

10. The method of claim 1, wherein the sample comprises exhaled breath from an individual.

11. The method of claim 1, wherein the sample is selected from the group consisting of sputum, blood, urine, pleural fluid, and pleural biopsy tissue.

12. The method of claim 1, wherein an amount of one or more volatile organic compounds is detected.

13. The method of claim 1, wherein the detecting is performed using a differential mobility spectrometer.

14. A method for identifying Klebsiella pneumonia bacteria in a sample, the method comprising:

a. collecting a sample suspected of comprising Klebsiella pneumonia bacteria; and

b. detecting one or more volatile organic compounds indicative of a presence of or response to treatment or resistance of the Klebsiella pneumonia bacteria in the sample, the one or more volatile organic compounds being selected from the group consisting of methanethiol, 2-heptanone, 2-nonanone, and 2-undecanone.

15. The method of claim 14, wherein the one or more volatile organic compounds comprises methanethiol.

16. The method of claim 14, wherein the one or more volatile organic compounds comprises 2-heptanone.

17. The method of claim 14, wherein the one or more volatile organic compounds comprises 2-nonanone.

18. The method of claim 14, wherein the one or more volatile organic compounds comprises 2-undecanone.

19. The method of claim 14, wherein a combination of two or more volatile organic compounds is indicative of the presence of or response to treatment or resistance of Klebsiella pneumonia in the sample.

20. The method of claim 14, wherein the sample was exposed to a candidate therapy for treating Klebsiella pneumonia.

21. The method of claim 14, wherein the sample comprises exhaled breath from an individual.

22. The method of claim 14, wherein the sample is selected from the group consisting of sputum, blood, urine, pleural fluid, and pleural biopsy tissue.

23. The method of claim 14, wherein an amount of one or more volatile organic compounds is detected.

24. The method of claim 14, wherein the detecting is performed using a differential mobility spectrometer.

25. A method for identifying Escherichia coli bacteria in a sample, the method comprising:

a. collecting a sample suspected of comprising Escherichia coli bacteria; and

b. detecting one or more volatile organic compounds indicative of a presence of or response to treatment or resistance of Escherichia coli bacteria in the sample, the one or more volatile organic compounds being selected from the group consisting of methanethiol and dimethyl disulfide.

26. The method of claim 25, wherein the one or more volatile organic compounds comprises methanethiol.

27. The method of claim 25, wherein the one or more volatile organic compounds comprises dimethyl disulfide.

28. The method of claim 25, wherein a combination of two or more volatile organic compounds is indicative of the presence of or response to treatment or resistance of Escherichia coli in the sample.

29. The method of claim 25, wherein the sample was exposed to a candidate therapy for treating Escherichia coli.

30. The method of claim 25, wherein the sample comprises exhaled breath from an individual.

31. The method of claim 25, wherein the sample is selected from the group consisting of sputum, blood, urine, pleural fluid, and pleural biopsy tissue.

32. The method of claim 25, wherein an amount of one or more volatile organic compounds is detected.

33. The method of claim 25, wherein the detecting is performed using a differential mobility spectrometer.