PLASMODIUM MALARIAE AND PLASMODIUM OVALE GENES AND USES THEREOF
First Claim
Patent Images
1. An isolated nucleic acid sequence or fragment thereof comprising or complementary to a nucleic acid sequence encoding a polypeptide, wherein the amino acid sequence of said polypeptide has at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1.
1 Assignment
0 Petitions
Accused Products
Abstract
The subject invention relates to nucleic acid sequences and amino acid sequences encoded thereby, derived from the Merozoite Surface Protein (MSP1) gene of the Plasmodium species P. malariae and P. ovale. Such genes and proteins have many beneficial diagnostic as well as therapeutic uses.
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Citations
44 Claims
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1. An isolated nucleic acid sequence or fragment thereof comprising or complementary to a nucleic acid sequence encoding a polypeptide, wherein the amino acid sequence of said polypeptide has at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1. - View Dependent Claims (5, 7)
- 1, amino acids 1634-1733 of SEQ ID NO;
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2. An isolated nucleic acid sequence or fragment thereof comprising or complementary to a nucleic acid sequence having at least 70% identity to a nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 2, nucleotides 62-5317 of SEQ ID NO;
2, nucleotides 4961-5260 of SEQ ID NO;
2, nucleotides 4181-4960 of SEQ ID NO;
2 and nucleotides 4181-5260 of SEQ ID NO;
2.
- 2, nucleotides 62-5317 of SEQ ID NO;
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3. An isolated nucleic acid sequence or fragment thereof comprising or complementary to a nucleic acid sequence encoding a polypeptide, wherein the amino acid sequence of said polypeptide has at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3. - View Dependent Claims (6)
- 3, amino acids 1614-1712 of SEQ ID NO;
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4. An isolated nucleic acid sequence or fragment thereof comprising or complementary to a nucleic acid sequence having at least 70% identity to a nucleic acid sequence comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 4, nucleotides 10-5202 of SEQ ID NO;
4, nucleotides 4849-5145 of SEQ ID NO;
4, nucleotides 4048-4848 of SEQ ID NO;
4 and nucleotides 4048-5145 of SEQ ID NO;
4.
- 4, nucleotides 10-5202 of SEQ ID NO;
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8. A purified protein or fragment thereof comprising an amino acid sequence having at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1.
- 1, amino acids 1634-1733 of SEQ ID NO;
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9. A purified protein or fragment thereof comprising an amino acid sequence having at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
- 3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3.
- 3, amino acids 1614-1712 of SEQ ID NO;
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10. A method of producing a protein comprising the steps of:
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(a) isolating a nucleic acid sequence comprising the nucleotide sequence of SEQ ID NO;
2;(b) constructing a vector comprising the isolated nucleic acid sequence operably linked to a regulatory sequence; and (c) introducing said vector into a host cell for a time and under conditions sufficient for expression of said protein. - View Dependent Claims (12)
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11. A method of producing a protein comprising the steps of:
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(a) isolating a nucleic acid sequence comprising the nucleotide sequence of SEQ ID NO;
4;(b) constructing a vector comprising the isolated nucleic acid sequence operably linked to a regulatory sequence; and (c) introducing said vector into a host cell for a time and under conditions sufficient for expression of said protein.
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13. A vector comprising a nucleic acid sequence comprising SEQ ID NO:
- 2 or SEQ ID NO;
4, operably linked to a regulatory sequence. - View Dependent Claims (14)
- 2 or SEQ ID NO;
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15. A method of detecting antibodies to P. malariae in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting the test sample with an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, for a time and under conditions sufficient for the formation of antibody/antigen complexes; and(b) detecting the presence of antibodies present in said test sample by detecting presence of said antibody/antigen complexes.
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16. A method of detecting antibodies to P. malariae in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, for a time and under conditions sufficient to allow for the formation of antibody/antigen complexes;(b) adding a conjugate to resulting antibody/antigen complexes for a time and under conditions sufficient to allow the conjugate to bind to bound antibody, wherein said conjugate comprises an antibody attached to a signal generating compound capable of generating a detectable signal; and (c) detecting the presence of antibodies present in said test sample by detecting presence of said signal generated by said signal generating compound.
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17. A method of detecting antibodies to P. malariae in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, for a time and under conditions sufficient to allow for the formation of antibody/antigen complexes;(b) adding a conjugate to resulting antibody/antigen complexes for a time and under conditions sufficient to allow the conjugate to bind to bound antibody, wherein said conjugate comprises an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, attached to a signal generating compound capable of generating a detectable signal; and(c) detecting the presence of antibodies present in said test sample by detecting presence of said signal generated by said signal generating compound.
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18. A method of detecting antibodies to P. ovale in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, for a time and under conditions sufficient for the formation of antibody/antigen complexes; and(b) detecting the presence of antibodies present in said test sample by detecting presence of said antibody/antigen complexes.
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19. A method of detecting antibodies to P. ovale in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, for a time and under conditions sufficient to allow for the formation of antibody/antigen complexes;(b) adding a conjugate to resulting antibody/antigen complexes for a time and under conditions sufficient to allow said conjugate to bind to bound antibody, wherein said conjugate comprises an antibody attached to a signal generating compound capable of generating a detectable signal; and c) detecting presence of antibodies present in said test sample by detecting presence of said signal generated by said signal generating compound.
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20. A method of detecting antibodies to P. ovale in a test sample suspecting of containing said antibodies comprising the steps of:
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(a) contacting said test sample with an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, for a time and under conditions sufficient to allow for the formation of antibody/antigen complexes;(b) adding a conjugate to resulting antibody/antigen complexes for a time and under conditions sufficient to allow the conjugate to bind to bound antibody, wherein said conjugate comprises an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, attached to a signal generating compound capable of generating a detectable signal; and(c) detecting the presence of antibodies present in said test sample by detecting presence of said signal generated by said signal generating compound.
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21. A method of detecting P. malariae antibodies in a test sample suspecting of containing said antibodies comprising the steps of:
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(a) contacting said test sample with anti-antibody, wherein said anti-antibody is raised against an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1 for a time and under conditions sufficient to allow for the formation of anti-antibody/P. malariae antibody complexes; and(b) detecting the presence of P. malariae antibodies which may be present in the test sample by detecting the presence of said anti-antibody/P. malariae antibody complexes.
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22. A method of detecting P. malariae antibodies in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with anti-antibody for a time and under conditions sufficient to allow for formation of anti-antibody/P. malariae antibody complexes; (b) adding a conjugate to the resulting anti-antibody/P. malariae antibody complexes for a time and under conditions sufficient to allow the conjugate to bind to the bound antibody, wherein said conjugate comprises a P. malariae antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, attached to a signal generating compound capable of generating a detectable signal; and(c) detecting the presence of antibodies which may be present in said test sample by detecting presence of said signal generated by said signal generating compound.
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23. A method of detecting antibodies to P. ovale in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with anti-antibody, wherein said anti-antibody is raised against an antibody raised against an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, for a time and under conditions sufficient to allow for formation of anti-antibody/P. ovale antibody complexes; and(b) detecting the presence of P. ovale antibodies which may be present in said test sample by detecting presence of said anti-antibody/P. ovale antibody complexes.
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24. A method of detecting antibodies to P. ovale in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with anti-antibody, for a time and under conditions sufficient to allow for formation of anti-antibody/P. ovale antibody complexes; (b) adding a conjugate to resulting anti-antibody/P. ovale antibody complexes for a time and under conditions sufficient to allow the conjugate to bind to the bound antibody, wherein the conjugate comprises a P. ovale antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3; and(c) detecting the presence of antibodies which may be present in said test sample by detecting presence of said signal generated by said signal generating compound.
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25. A method of detecting the presence of P. malariae antibodies in a test sample suspecting of containing said antibodies comprising the steps of:
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(a) contacting said test sample with anti-antibody for a time and under conditions sufficient to allow for formation of anti-antibody/P. malariae antibody complexes; (b) adding antigen to the resulting anti-antibody/P. malariae antibody complexes for a time and under conditions sufficient to allow said antigen to bind to bound antibody, wherein said antigen comprises an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1;(c) adding a conjugate to the resulting anti-antibody/P. malariae antibody/antigen complexes, wherein said conjugate comprises a composition comprising a monoclonal or polyclonal antibody attached to a signal generating compound capable of detecting a detectable signal; and (d) detecting presence of antibodies which may be present in said test sample by detecting presence of said signal generated by said signal generating compound.
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26. A method of detecting antibodies to P. ovale in a test sample suspected of containing said antibodies comprising the steps of:
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(a) contacting said test sample with anti-antibody wherein said anti-antibody is raised against an antibody raised against an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, for a time and under conditions sufficient to allow for formation of anti-antibody/P. ovale antibody complexes;(b) adding an antigen to the resulting anti-antibody/P. ovale antibody complexes for a time and under conditions sufficient to allow the antigen to bind to the bound antibody; (c) adding a conjugate to the resulting anti-antibody/P. ovale antibody/antigen complexes, wherein said conjugate comprises a composition comprising a monoclonal or polyclonal antibody attached to a signal generating compound capable of detecting a detectable signal; and (d) detecting the presence of antibodies which may be present in said test sample by detecting presence of said signal generated by said signal generating compound.
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27. A method of detecting antibodies to P. malariae, P. falciparum, P. vivax and P. ovale in a test sample suspected of containing at least one of said antibodies comprising the steps of:
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(a) contacting said test sample with;
1) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1,
2) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3,
3) an antigen of P. falciparum and
4) an antigen of P. vivax, for a time and under conditions sufficient for the formation of P. malariae antibody/antigen complexes, P. falciparum antibody/antigen complexes, P. vivax antibody/antigen complexes and P. ovale antibody/antigen complexes; and(b) detecting the presence of antibodies present in said test sample by detecting presence of one or more of said complexes.
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28. A method of detecting antibodies to P. malariae, P. ovale, P. vivax and P. falciparum in a test sample suspected of containing at least one of said antibodies comprising the steps of:
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(a) contacting said test sample with;
1) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1,
2) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3,
3) a P. vivax antigen and
4) a P. falciparum antigen, for a time and under conditions sufficient to allow for the formation of P. malariae antibody/antigen complexes, P. ovale antibody/antigen complexes, P. vivax antibody/antigen complexes and P. falciparum antibody/antigen complexes;(b) adding four conjugates to the resulting antibody/antigen complexes for a time and under conditions sufficient to allow each conjugate to bind to bound antibody, wherein a first conjugate comprises an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, attached to a signal generating compound capable of generating a detectable signal
2) a second conjugate comprises an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, attached to a signal generating compound capable of generating a detectable signal,
3) a third conjugate comprises a P. vivax antigen attached to a signal generating signal capable of generating a detectable signal and
4) a fourth conjugate comprises a P. falciparum antigen attached to a signal generating compound capable of generating a detectable signal; and(c) detecting the presence of antibody to P. malariae, P. ovale, P. vivax and P. falciparum which may be present in the test sample by detecting presence of said signal generated by said signal generating compound.
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29. A method of detecting antibodies to P. malariae, P. ovale, P. vivax and P. falciparum in a test sample suspected of containing at least one of said antibodies comprising the steps of:
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(a) contacting said test sample with an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1,
2) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3,
3) a P. vivax antigen and
4) a P. falciparum antigen, for a time and under conditions sufficient to allow for the formation of P. malariae antibody/antigen complexes, P. ovale antibody/antigen complexes, P. vivax antibody/antigen complexes and P. falciparum antibody/antigen complexes;(b) adding a conjugate to the resulting antibody/antigen complexes for a time and under conditions sufficient to allow each conjugate to bind to bound antibody, wherein said conjugate comprises an antibody attached to a signal generating compound capable of generating a detectable signal; and (c) detecting the presence of antibody to P. malariae, P. ovale, P. vivax and P. falciparum antibody which may be present in said test sample by detecting presence of said signal generated by said signal generating compound.
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30. A method for detecting the presence of antibodies to P. malariae, P. ovale, P. vivax and P. falciparum in a test sample suspected of containing at least one of said antibodies comprising the steps of:
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(a) contacting the test sample with;
1) anti-antibody, wherein said anti-antibody is raised against an antibody raised against an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1,
2) anti-antibody, wherein said anti-antibody is raised against an antibody raised against an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3,
3) anti-antibody specific for P. vivax and
4) anti-antibody specific for P. falciparum, for a time and under conditions sufficient to allow for the formation of anti-antibody/P. malariae antibody complexes, anti-antibody/P. ovale antibody complexes, anti-antibody/P. vivax antibody complexes and
4) anti-antibody/P. falciparum antibody complexes; and(b) detecting the presence of antibody to P. malariae, P. ovale, P. vivax and P. falciparum which may be present in the test sample by detecting presence of one or more of said complexes.
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31. A method for detecting the presence of antibodies to P. malariae, P. ovale, P. vivax and P. falciparum in a test sample suspected of containing at least one of said antibodies comprising the steps of:
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(a) contacting the test sample with anti-antibody to allow for the formation of anti-antibody/antibody complexes; (b) adding a first conjugate, a second conjugate, a third conjugate and a fourth conjugate to resulting anti-antibody/antibody complexes for a time and under conditions sufficient to allow the conjugates to bind to bound antibody, wherein said first conjugate comprises an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, attached to a signal generating compound capable of generating a detectable signal, wherein said second conjugate comprises an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, attached to a signal generating compound capable of generating a detectable signal, wherein said third conjugate comprises a P. vivax antigen attached to a signal generating compound capable of generating a detectable signal and wherein said fourth conjugate comprises a P. falciparum antigen attached to a signal generating compound capable of generating a detectable signal; and(c) detecting the presence of antibodies to P. malariae, P. ovale, P. vivax and P. falciparum in the test sample by detecting presence of said signal generated by said signal generating compound.
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32. A vaccine comprising:
- a) at least one antigen selected from the group consisting of;
1) an antigen comprising the amino acid sequence of SEQ ID NO;
1 or an epitope thereof,
2) an antigen comprising the amino acid sequence of SEQ ID NO;
3 or an epitope thereof,
3) a P. falciparum antigen and
4) a P. vivax antigen and b) a pharmaceutically acceptable adjuvant.
- a) at least one antigen selected from the group consisting of;
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33. A vaccine comprising:
- a) an antibody raised against at least one antigen selected from the group consisting of;
1) an antigen comprising the amino acid sequence of SEQ ID NO;
1 or an epitope thereof,
2) an antigen comprising the amino acid sequence of SEQ ID NO;
3 or an epitope thereof,
3) a P. falciparum antigen and
4) a P. vivax antigen and b) a pharmaceutically acceptable adjuvant.
- a) an antibody raised against at least one antigen selected from the group consisting of;
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34. A kit for determining the presence of antibody to P. malariae in a test sample comprising:
- a) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1 and b) a conjugate comprising an antibody attached to a signal-generating compound capable of generating a detectable signal.
- a) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
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35. A kit for determining the presence of antibody to P. ovale in a test sample comprising:
- a) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3 and b) a conjugate comprising an antibody attached to a signal-generating compound capable of generating a detectable signal.
- a) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
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36. A kit for determining the presence of antibody to P. malariae, P. ovale, P. vivax and P. falciparum in a test sample comprising:
- a) i) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, ii) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, iii) a P. vivax antigen and iv) a P. falciparum antigen and b) a conjugate comprising an antibody attached to a signal generating compound capable of generating a detectable signal.
- a) i) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
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37. A kit for detecting antibodies to P. malariae in a test sample comprising:
- a) an anti-antibody and b) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1.
- a) an anti-antibody and b) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
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38. A kit for detecting antibodies to P. malariae in a test sample comprising:
- a) an anti-antibody and b) a conjugate comprising an antigen, wherein said antigen comprises an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1 and wherein the conjugate is attached to a signal-generating compound capable of generating a detectable signal.
- a) an anti-antibody and b) a conjugate comprising an antigen, wherein said antigen comprises an amino acid sequence selected from the group consisting of SEQ ID NO;
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39. A kit for detecting antibodies to P. ovale in a test sample comprising:
- a) an anti-antibody and b) an antigen comprising an amino acid sequence selected from the group consisting of comprises an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3.
- a) an anti-antibody and b) an antigen comprising an amino acid sequence selected from the group consisting of comprises an amino acid sequence selected from the group consisting of SEQ ID NO;
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40. A kit for detecting antibodies to P. ovale in a test sample comprising:
- a) an anti-antibody and b) a conjugate comprising an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, wherein said conjugate is attached to a signal-generating compound capable of generating a detectable signal.
- a) an anti-antibody and b) a conjugate comprising an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
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41. A kit for detecting antibodies to P. malariae, P. ovale, P. vivax and P. falciparum in a test sample comprising:
- a) an anti-antibody and b) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, a P. vivax antigen and a P. falciparum antigen
- a) an anti-antibody and b) an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
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42. A kit for detecting antibodies to P. malariae, P. ovale, P. vivax and P. falciparum in a test sample comprising:
- a) an anti-antibody and b) a first conjugate comprising an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
1, amino acids 1634-1733 of SEQ ID NO;
1, amino acids 1374-1633 of SEQ ID NO;
1 and amino acids 1374-1733 of SEQ ID NO;
1, attached to a signal generating compound capable of generating a detectable signal, a second conjugate comprising an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
3, amino acids 1614-1712 of SEQ ID NO;
3, amino acids 1347-1613 of SEQ ID NO;
3 and amino acids 1347-1712 of SEQ ID NO;
3, attached to a signal generating compound capable of generating a detectable signal, a third conjugate comprising a P. vivax antigen attached to a signal generating compound capable of generating a detectable signal and a fourth conjugate comprising a P. falciparum antigen attached to a signal generating compound capable of generating a detectable signal.
- a) an anti-antibody and b) a first conjugate comprising an antigen comprising an amino acid sequence selected from the group consisting of SEQ ID NO;
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43. A purified protein or fragment thereof comprising an amino acid sequence having at least 70% sequence identity to SEQ ID NO:
- 47 or SEQ ID NO;
48.
- 47 or SEQ ID NO;
-
44. An isolated nucleic acid molecule or fragment thereof comprising a nucleotide sequence having at least 70% sequence identity to SEQ ID NO:
- 43 or SEQ ID NO;
44.
- 43 or SEQ ID NO;
Specification