METHOD OF CULTURING AGARICUS BISPORUS MYCELIUM AND MEDIUM FOR CULTURING THE SAME

• US 20090235579A1
• Filed: 05/25/2007
• Published: 09/24/2009
• Est. Priority Date: 05/25/2006
• Status: Abandoned Application

• Current Assignee: CJ Corporation (CJ Group)
• Original Assignee: CJ Corporation (CJ Group)
• Inventors: Kim, Yong-Hwi, Kim, Young-Duk
• Application Number:
  US12/302,469
• Publication Number:
  US 20090235579A1
• Time in Patent Office: Days
• US Class Current: 47/1.100
• CPC Class Codes: A01G 18/20 Culture media, e.g. compost
• CPC Class Codes: A01G 18/40 Cultivation of spawn

Abstract

Provided are a method of culturing Agaricus bisporus mycelium, the method comprising culturing Agaricus bisporus mycelium or spores thereof in a liquid medium comprising sugar cane extract, and a medium for culturing the Agaricus bisporus mycelium.

Claims

1. A method of culturing Agaricus bisporus mycelium, comprising culturing Agaricus bisporus mycelium or spores thereof in a liquid medium comprising sugar cane extract.

2. The method of claim 1, wherein the sugar cane extract has a concentration of 10-30 g/l.

3. The method of claim 1, wherein the medium further comprises sodium nitrate as a nitrogen source.

4. The method of claim 3, wherein the sodium nitrate has a concentration of 1 -10 g/l.

5. The method of claim 1, wherein the medium comprises yeast extract in a concentration of 1-10 g/l.

6. The method of claim 1, wherein the culturing of Agaricus bisporus mycelium is conducted at an initial pH of 6.0 to 6.5, at a culture temperature 25-28°

C., while stirring at a stirring velocity of 150-250 rpm.

7. The method of claim 1, wherein the Agaricus bisporus mycelium is dispersed using a blender.

8. The method of claim 1, wherein the culturing of Agaricus bisporus mycelium is conducted for 3-6 days before completion.

9. The method of claim 1, further comprising preparing a mushroom seed culture by pre-culturing the Agaricus bisporus mycelium or spores thereof in a liquid medium comprising potato dextrose broth of 15-25 g/l, yeast extract of 1-10 g/l, malt extract of 2-5 g/l, and soytone of 2-5 g/l.

10. The method of claim 9, wherein the pre-culturing of the Agaricus bisporus mycelium or spores thereof is conducted at an initial pH of 6.0 to 6.5, at a culture temperature 25-28°

C., while stirring at a stirring velocity of 150-250 rpm.

11. The method of claim 10, further comprising dispersing the pre-cultured mushroom mycelia using a blender.

12. A medium for culturing Agaricus bisporus mycelium, comprising sugar cane extract of 10-30 g/l and sodium nitrate of 1-10 g/l.

13. The medium of claim 12, further comprising yeast extract in a concentration of 1-10 g/l.

14. A medium for pre-culturing Agaricus bisporus mycelium or spores thereof, comprising potato dextrose broth of 15-25 g/l, yeast extract of 1-10 g/l, malt extract of 2-5 g/l, and soytone of 2-5 g/l.