Electrochemical Determination of Factor XA Inhibitors
First Claim
1. A method for determining a factor Xa inhibitor in a blood sample comprising:
- (a) contacting a blood sample with a known amount of factor X reagent, an activator reagent that induces the conversion of factor X into factor Xa, and a detection reagent comprising a peptidic thrombin substrate that can be cleaved by thrombin and comprising a carboxyl end that is amidically bound to an electrogenic substance,(b) measuring the amount or activity of the electrogenic substance that is cleaved from the thrombin substrate by measuring an electrochemical signal associated with the amount or activity of the electrogenic substance;
(c) relating the amount or activity of the electrogenic substance to the amount of Xa inhibitor in the sample, thereby determining the amount of the factor Xa inhibitor in the sample.
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Accused Products
Abstract
Methods and devices for determining factor Xa inhibitors, in particular heparins and fractionated or low-molecular-weight heparins, as well as direct factor Xa inhibitors in blood samples. The methods include contacting a blood sample with a detection reagent that contains at least one thrombin substrate having a peptide residue that can be cleaved by thrombin and is amidically bound via the carboxyl end to an electrogenic substance, and with a known amount of factor X reagent and an activator reagent which induces the conversion of factor X into factor Xa. Subsequently, in a second step, the amount or activity of the electrogenic substance that is cleaved from the thrombin substrate by the factor Xa-mediated thrombin activation and/or the time course thereof is determined as the measurement signal using electrochemical methods. In a third step, the amount of the factor Xa inhibitor in the sample of the blood to be analyzed or a measured quantity that correlates therewith, in particular a clotting time that correlates therewith, is determined on the basis of this measurement signal.
22 Citations
21 Claims
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1. A method for determining a factor Xa inhibitor in a blood sample comprising:
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(a) contacting a blood sample with a known amount of factor X reagent, an activator reagent that induces the conversion of factor X into factor Xa, and a detection reagent comprising a peptidic thrombin substrate that can be cleaved by thrombin and comprising a carboxyl end that is amidically bound to an electrogenic substance, (b) measuring the amount or activity of the electrogenic substance that is cleaved from the thrombin substrate by measuring an electrochemical signal associated with the amount or activity of the electrogenic substance; (c) relating the amount or activity of the electrogenic substance to the amount of Xa inhibitor in the sample, thereby determining the amount of the factor Xa inhibitor in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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- 14. A test element for determining a factor Xa inhibitor, the element comprising an inert carrier comprising at least two electrodes, a factor X reagent, an activator reagent for converting factor X into factor Xa, and a detection reagent that contains at least one peptidic thrombin substrate that can be cleaved by thrombin and comprising a carboxyl end that is amidically bound to an electrogenic substance.
Specification