DLC-1 GENE DELETED IN CANCERS
First Claim
1. An isolated nucleic acid molecule selected from the group consisting of:
- (a) nucleic acid molecules encoding an amino acid sequence as shown in Seq. I.D. No. 2;
(b) nucleic acid molecules which hybridize under stringent conditions to a nucleic acid molecule according to (a), and which encode a protein having an activity of the DLC-1 protein;
(c) nucleic acid molecules comprising a nucleotide sequence as shown in Seq. I.D. No. 1.
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Abstract
A cDNA molecule corresponding to a newly discovered human gene is disclosed. The new gene, which is frequently deleted in liver cancer cells and cell lines, is called the DLC-1 gene. Because the gene is frequently deleted in liver cancer cells, but present in normal cells, it is thought to act as a tumor suppressor. This gene is also frequently deleted in breast and colon cancers, and its expression is decreased or undetectable in many prostate and colon cancers. Also disclosed is the amino acid sequence of the protein encoded by the DLC-1 gene. Methods of using these biological materials in the diagnosis and treatment of hepatocellular cancer, breast cancer, colon cancer, prostate cancer, and adenocarcinomas are presented.
11 Citations
17 Claims
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1. An isolated nucleic acid molecule selected from the group consisting of:
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(a) nucleic acid molecules encoding an amino acid sequence as shown in Seq. I.D. No. 2; (b) nucleic acid molecules which hybridize under stringent conditions to a nucleic acid molecule according to (a), and which encode a protein having an activity of the DLC-1 protein; (c) nucleic acid molecules comprising a nucleotide sequence as shown in Seq. I.D. No. 1. - View Dependent Claims (2, 3)
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- 4. An isolated nucleic acid molecule comprising at least 15 consecutive nucleotides of a DNA sequence shown in Seq. I.D. No. 1.
- 10. An isolated cDNA molecule encoding a human DLC-1 protein.
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15. A purified protein having DLC-1 protein biological activity, and comprising an amino acid sequence selected from the group of:
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(a) the amino acid sequence shown in Seq. I.D. No.2; (b) amino acid sequences that differ from those specified in (a) by one or more conservative amino acid substitutions; and (c) amino acid sequences having at least 90% sequence identity to the sequence specified in (a). - View Dependent Claims (7, 8, 9, 13, 16, 17)
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Specification