Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
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Abstract
This invention provides methods for covalently affixing a biomolecule to either a second molecule or a solid surface using 1,3-dipolar cycloaddition chemistry. This invention also provides related methods and compositions.
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Citations
95 Claims
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1-80. -80. (canceled)
- 81. A method for forming a covalent bond between a biomolecule labeled with an azido moiety and a fluorescent dye labeled with an alkynyl moiety which comprises contacting the azido-labeled biomolecule with the alkynyl-labeled fluorescent dye under conditions permitting a 1,3-dipolar cycloaddition reaction to occur between the azido moiety and the alkynyl moiety so as to thereby form a covalent bond between the azido-labeled biomolecule and the alkynyl-labeled fluorescent dye, wherein the azido-labeled biomolecule is an azido-labeled amino acid, protein, sugar or nucleic acid.
- 83. A method for forming a covalent bond between a biomolecule labeled with an azido moiety and a biotin labeled with an alkynyl moiety which comprises contacting the azido-labeled biomolecule with the alkynyl-labeled biotin under conditions permitting a 1,3-dipolar cycloaddition reaction to occur between the azido moiety and the alkynyl moiety so as to thereby form a covalent bond between the azido-labeled biomolecule and the alkynyl-labeled biotin, wherein the azido-labeled biomolecule is an azido-labeled amino acid, protein, sugar or nucleic acid.
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94. A biomolecule covalently bonded to a fluorescent dye via a 1,2,3-triazole ring, wherein the biomolecule is an amino acid, a protein, a sugar, or a nucleic acid.
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95. A biomolecule covalently bonded to a biotin via a 1,2,3-triazole ring, wherein the biomolecule is an amino acid, a protein, a sugar, or a nucleic acid.
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