×

Methods and Assays for Capture of Nucleic Acids

  • US 20090246788A1
  • Filed: 03/20/2009
  • Published: 10/01/2009
  • Est. Priority Date: 04/01/2008
  • Status: Abandoned Application
First Claim
Patent Images

1. A method for capturing target nucleic acid sequences comprising:

  • a) providing;

    i) a nucleic acid sample wherein said nucleic acid sample may or may not comprise a target sequence,ii) at least one flap endonuclease and at least one ligase, andiii) a plurality of oligonucleotide probes, wherein said probes comprise target sequences and a hairpin structure,b) applying said nucleic acid sample to said oligonucleotide probes under conditions wherein hybridization is allowed to occur between target sequences and probes; and

    c) applying at least one flap endonuclease and at least one ligase to the hybridized nucleic acid/probes complex under conditions wherein enzymatic reactions are allowed to occur thereby capturing a nucleic acid target sequence.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×