ENGINEERED FLUORESCENT DYE LABELED NUCLEOTIDE ANALOGS FOR DNA SEQUENCING
First Claim
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1. A nucleotide composition, comprising the structure:
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N—
Y—
Fwherein N is a nucleoside polyphosphate or analog thereof;
F is a detectable label moiety; and
Y comprises a polymerase interacting linkage complex.
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Abstract
Engineered nucleotide compositions, having polymerase interacting components that improve the interactivity of the polymerase and the nucleotide, particularly for nucleic acid sequencing applications. Compositions include the interactive polymerases along with the nucleotide analogs. Kits, methods and systems are provided for analysis of nucleic acid synthesis reactions.
28 Citations
17 Claims
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1. A nucleotide composition, comprising the structure:
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N—
Y—
Fwherein N is a nucleoside polyphosphate or analog thereof; F is a detectable label moiety; and Y comprises a polymerase interacting linkage complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A nucleotide composition, comprising the structure:
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N—
Y—
Fwhere N is a nucleoside polyphosphate group or analog thereof coupled through a phosphate group other than an alpha phosphate group; L is a detectable labeling group; and Y comprises a polymerase interacting linkage complex having a polymerase interacting component selected from phenyl, biphenyl, triphenyl and a coumarin derivative. - View Dependent Claims (10)
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11. A nucleotide composition, comprising the structure:
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N-L1-P-L2-Fwherein N is a nucleoside polyphosphate or analog thereof, L1 is a first linker group, P is a polymerase interacting group, L2 is a second linker group, and F is a detectable label moiety.
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12. A method of monitoring polymerase mediated, template dependent primer extension, comprising:
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providing a polymerase template primer complex; contacting the complex with a nucleotide composition, comprising the structure;
N—
Y—
Fwherein N is a nucleoside polyphosphate or analog thereof; F is a detectable label moiety; and Y comprises a polymerase interacting linkage complex; and monitoring reaction of the nucleotide composition with the complex in a primer extension reaction by detecting a signal from the detectable label. - View Dependent Claims (13, 14, 15, 16, 17)
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Specification