×

FOCUSED LIBRARY GENERATION

  • US 20090247426A1
  • Filed: 03/30/2009
  • Published: 10/01/2009
  • Est. Priority Date: 03/31/2008
  • Status: Abandoned Application
First Claim
Patent Images

1. A method of producing a library of mutated nucleic acids, said method comprising:

  • providing a single-stranded template nucleic acid comprising a first sequence of nucleotides;

    providing a first set of primers, wherein a majority of said first set of primers are perfectly complementary to at least a portion of said first sequence of nucleotides;

    providing a second set of primers, wherein a majority of said second set of primers are complementary to at least a portion of said first sequence of nucleotides except for at least one pre-selected mismatched nucleotide complementary to at least one targeted mutation;

    combining said template nucleic acid with said first set of primers and said second set of primers under conditions suitable for amplification of said template nucleic acid; and

    amplifying said template nucleic acid to produce said library of mutated nucleic acids, wherein said mutated nucleic acids have at least one targeted mutation therein.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×