FLUORESCENT SIGNAL ANALYZING APPARATUS AND FLUORESCENT SIGNAL ANALYZING METHOD

• US 20090252414A1
• Filed: 06/17/2009
• Published: 10/08/2009
• Est. Priority Date: 01/16/2007
• Status: Active Grant

• Current Assignee: Olympus Corporation
• Original Assignee: Olympus Corporation
• Inventors: SUZUKI, Akemi
• Granted Patent: US 8,194,981 B2
• Time in Patent Office: Days
• US Class Current: 382/170
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging

Abstract

A fluorescent signal analyzing apparatus includes an image input unit, a storage unit, an extracting unit, and an analyzing unit. At least one fluorescent image of a sample is input to the input unit. The storage unit stores data on the at least one fluorescent image input to the image input unit. The extracting unit extracts, for each of the fluorescent images, data on a fluorescence intensity in a desired region on the at least one fluorescent image from the data on the at least one fluorescent image stored in the storage unit. The analyzing unit calculates at least one of a correlation and a photon counting histogram for the desired region by use of the data on the fluorescence intensity.

Claims

1. A fluorescent signal analyzing apparatus comprising:

an image input unit to which at least one fluorescent image of a sample is input;

a storage unit to store data on the at least one fluorescent image input to the image input unit;

an extracting unit to extract, for each of the fluorescent images, data on a fluorescence intensity in a desired region on the at least one fluorescent image from the data on the at least one fluorescent image stored in the storage unit; and

an analyzing unit to calculate at least one of a correlation and a photon counting histogram for the desired region by use of the data on the fluorescence intensity.

2. The fluorescent signal analyzing apparatus according to claim 1, wherein a plurality of fluorescent images of the sample repetitively captured at predetermined time intervals are input to the image input unit;

the storage unit stores data on the plurality of fluorescent images; and

the extracting unit extracts, for each of the fluorescent images, data on fluorescence intensities in desired regions on the plurality of fluorescent images from the data on the plurality of fluorescent images stored in the storage unit.

3. The fluorescent signal analyzing apparatus according to claim 1, wherein the desired region is a pixel region including a single pixel.

4. The fluorescent signal analyzing apparatus according to claim 1, wherein the desired region is a pixel region including a plurality of pixels.

5. The fluorescent signal analyzing apparatus according to claim 4, wherein the data on the fluorescence intensities is a statistic of the fluorescence intensities of the plurality of pixels.

6. The fluorescent signal analyzing apparatus according to claim 5, wherein the statistic is one of an integrated value, an average value, a maximum value, a minimum value, a relative difference, and an absolute difference of the fluorescence intensities of the plurality of pixels.

7. The fluorescent signal analyzing apparatus according to claim 1, wherein the fluorescent image is composed of distributions of the fluorescence intensities.

8. The fluorescent signal analyzing apparatus according to claim 1, wherein the analyzing unit calculates a diffusion time by further using the predetermined time intervals.

9. The fluorescent signal analyzing apparatus according to claim 1, further comprising region a specifying unit to specify the desired region.

10. The fluorescent signal analyzing apparatus according to claim 1, further comprising a display unit to display, by at least one of luminance, a table, a graph, and a numerical value, a result calculated by the analyzing unit

11. The fluorescent signal analyzing apparatus according to claim 1, further comprising:

a display unit to display at least one of the at least one of the fluorescent images stored in the storage unit; and

a region specifying unit to specify the desired region on the fluorescent image displayed on the display unit.

12. A fluorescent signal analyzing method comprising:

storing data on at least one fluorescent image of a sample;

extracting, for each of the fluorescent images, data on a fluorescence intensity in a desired region on the at least one fluorescent image from the stored data on the at least one fluorescent image; and

calculating at least one of a correlation and a photon counting histogram for the desired region by use of the extracted data on the fluorescence intensity.

13. The fluorescent signal analyzing method according to claim 12, wherein the storing includes storing data on a plurality of fluorescent images of the sample repetitively captured at predetermined time intervals, and the extracting includes extracting, for each of the fluorescent images, data on fluorescence intensities in desired regions on the plurality of fluorescent images from the stored data on the plurality of fluorescent images.

14. The fluorescent signal analyzing method according to claim 12, wherein the desired region is a pixel region including a single pixel.

15. The fluorescent signal analyzing method according to claim 12, wherein the desired region is a pixel region including a plurality of pixels.

16. The fluorescent signal analyzing method according to claim 15, wherein the data on the fluorescence intensities is a statistic of the fluorescence intensities of the plurality of pixels.

17. The fluorescent signal analyzing method according to claim 16, wherein the statistic is one of an integrated value, an average value, a maximum value, a minimum value, a relative difference and an absolute difference of the fluorescence intensities of the plurality of pixels.

18. The fluorescent signal analyzing method according to claim 12, wherein the fluorescent image is composed of distributions of the intensities of fluorescent light emitted from the sample in response to the application of excitation light.

19. The fluorescent signal analyzing method according to claim 12, further comprising calculating a diffusion time by using the predetermined time intervals.

20. The fluorescent signal analyzing method according to claim 12, further comprising displaying an analytic result by at least one of luminance, a table, a graph and a numerical value.

21. The fluorescent signal analyzing method according to claim 12, further comprising:

displaying at least one of the at least one of the fluorescent images; and

specifying the desired region on the displayed fluorescent image.