Novel Chemistry Used in Biosensors

• US 20090253149A1
• Filed: 10/17/2008
• Published: 10/08/2009
• Est. Priority Date: 10/17/2007
• Status: Active Grant

• Current Assignee: Ohmx Corporation
• Original Assignee: Ohmx Corporation
• Inventors: Eckermann, Amanda, Meade, Thomas J., Bertin, Paul A., Ahrens, Michael, Georganopoulou, Dimitra, Wunder, Markus Franz, Gray, Harry B.
• Granted Patent: US 8,734,631 B2
• Time in Patent Office: Days
• US Class Current: 435/7.400
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: C12Q 1/485 involving kinase
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence
• CPC Class Codes: Y10T 29/49117 Conductor or circuit manufacturing

Abstract

The invention relates to novel compositions and methods for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.

Claims

1. A composition comprising an electrode comprising a covalently attached electroactive complex (EAM), said EAM comprising a transition metal and at least one cyano ligand.

2. The composition according to claim 1, wherein said EAM comprises at least two cyano ligands.

3. The composition according to claim 1, wherein said EAM comprises at least three cyano ligands.

4. The composition according to claim 1, wherein said EAM comprises at least four cyano ligands.

5. The composition according to claim 1, wherein said EAM comprises at least five cyano ligands.

6. The composition according to claim 1, wherein said transition metal is osmium.

7. The composition according to claim 1, wherein said electrode is gold and said EAM is covalently attached to said electrode via a sulfur atom.

8. The composition according to claim 1, wherein said electrode is gold and said EAM is covalently attached to said electrode via two sulfur atoms.

13. The composition according to claim 1, wherein said electrode comprises a redox active capture complex (REAMC) comprising said EAM and a capture ligand.

21. The composition according to claim 1, wherein said electrode comprises a first species comprising said EAM and a second species comprising a capture ligand.

24. The composition according to claim 1, wherein said composition comprises an array of electrodes, each comprising a covalently attached transition metal complex comprising a transition metal and at least one cyano ligand.

9-12. -12. (canceled)

14-20. -20. (canceled)

22-23. -23. (canceled)

25. A method of detecting a target enzyme comprising:

a) contacting a sample with a composition comprising an electrode comprising;

i) an electroactive moiety (EAM) comprising a transition metal and at least one cyano ligand with a first E⁰;

ii) a capture ligand;

under conditions whereby said target enzyme, if present, alters said capture ligand such that said EAM has a second E⁰; and

b) measuring said second E⁰.

26. (canceled)

27. A method of detecting a target enzyme comprising:

a) contacting a sample with a composition comprising a REAMC comprising;

i) an electroactive moiety (EAM) comprising a transition metal and at least one cyano ligand with a first E⁰; and

ii) a capture ligand;

under conditions whereby said target enzyme, if present, alters said capture ligand such that said EAM has a second E⁰; and

b) measuring said second E⁰.

28. A composition comprising an electrode comprising:

a) a first species comprising a functional group; and

b) an electroactive complex (EAM), said EAM comprising a transition metal and at least one cyano ligand.

29. The composition according to claim 28, wherein said functional group is a maleimide group.

30. A method of making a biosensor comprising:

a) providing an electrode comprising;

i) a first species comprising a first functional group; and

ii) an electroactive complex (EAM), said EAM comprising a transition metal and at least one cyano ligand; and

b) contacting said electrode with a biomolecule comprising a second functional group to form a covalent bond between said first species and said biomolecule.

31-33. -33. (canceled)

34. A compound having the formula:

Anchor−

Spacer 1−

EAM−

(Spacer

     2)_n−

CL 

 

(I)

wherein said anchor comprises a cyclic-disulfide group,

EAM is an electroactive moiety comprises a solvent accessible redox compound,

CL is a capture ligand,

Spacer 1 is a SAM forming species, and

n=0 or 1.

35-42. -42. (canceled)

43. A composition comprising:

a) a first anchor;

b) a charge-neutralizing ligand linked to said first anchor;

c) an electroactive moiety (EAM) covalently attached to said charge-neutralizing ligand; and

d) a capture ligand.

44-45. -45. (canceled)

46. A composition comprising an electrode comprising a self-assembled monolayer (SAM), wherein said SAM comprises a compound having the formula:

Anchor−

Spacer 1−

EAM−

Spacer 2−

CL 

 

(II),

wherein said anchor is linked to said electrode group through a disulfide group,

EAM is an electroactive moiety comprises a solvent accessible redox compound,

CL is a capture ligand,

Spacer 1 is either insulating or conducting, and

Spacer 2 is optional.

47. A method, comprising:

a) providing a compound having the formula;

Anchor−

Spacer 1−

EAM−

(Spacer

     2)_n−

CL 

 

(I),

wherein said anchor comprises a cyclic-disulfide group,

EAM is an electroactive moiety comprises a solvent accessible redox compound,

CL is a capture ligand,

Spacer 1 is either insulating or conduction, and

n=0 or 1; and

b) contacting said compound to an electrode by opening said cyclic disulfide to form an attachment of said anchor to said electrode.

48. (canceled)

49. A composition comprising an array of electrode, each electrode comprising a compound having the formula:

Anchor−

Spacer 1−

EAM−

(Spacer

     2)_n−

CL 

 

(I),

wherein said anchor comprises a cyclic-disulfide group,

EAM is an electroactive moiety comprises a solvent accessible redox compound,

CL is a capture ligand,

Spacer 1 is either insulating or conduction, and

n=0 or 1.

50. A method of detecting a target analyte in a test sample, comprising:

a) providing an electrode comprises a compound having the formula;

Anchor−

Spacer 1−

EAM−

(Spacer

     2)_n−

CL 

 

(I),

wherein said anchor comprises a cyclic-disulfide group,

EAM is an electroactive moiety comprises a solvent accessible redox compound,

CL is a capture ligand,

Spacer 1 is either insulating or conduction, and

n=0 or 1; and

b) contacting said electrode with said test sample; and

c) determining the presence of said target analyte by measuring the reorganization energy of said EAM.