PREWETTING LATERAL FLOW TEST STRIP

• US 20090257915A1
• Filed: 04/20/2009
• Published: 10/15/2009
• Est. Priority Date: 03/30/2001
• Status: Active Grant

• Current Assignee: Relia Biotechnologies (Shenzhen) Ltd.
• Original Assignee: Relia Diagnostic Systems Inc.
• Inventors: Quan, Stella S., Polito, Alan J., Dinello, Robert K.
• Granted Patent: US 7,955,791 B2
• Time in Patent Office: Days
• US Class Current: 422/56
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/5055 Hinged, e.g. opposable surfaces
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 435/974 Aids related test
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function
• CPC Class Codes: Y10S 436/817 Steroids or hormones
• CPC Class Codes: Y10T 156/1089 of discrete laminae to single face of additional lamina

Abstract

A test strip and method for detecting an analyte present in a sample. The test strip comprising: a buffer addition zone to which a buffer may be added; an absorbent zone proximal to the buffer addition zone; one or more test zones distal to the buffer addition zone, at least one of the test zones including a first analyte binding agent immobilized therein which is capable of binding to the analyte to be detected; a terminal buffer flow zone distal to the one or more test zones, the absorbent zone being positioned relative to the buffer addition zone and having an absorption capacity relative to the other zones of the test strip such that when a volume of buffer within a predetermined buffer volume range for the test strip is added to the buffer addition zone, a distal diffusion front of the buffer diffuses from the buffer addition zone to a distal diffusion point within the terminal buffer flow zone and then diffuses proximal relative to the one or more test zones; and a sample addition zone distal to the terminal buffer flow zone to which a sample may be added.

Claims

1-63. -63. (canceled)

64. A test strip comprising:

a chromatographic strip with a first end and a second end;

an absorbent zone positioned at the first end of the chromatographic strip;

a buffer addition zone positioned near the first end of the chromatographic strip;

a sample addition zone positioned at or near the second end of the chromatographic strip; and

one or more test zones positioned on the chromatographic strip between the buffer addition zone and the sample addition zone.

68. A method of making a test strip comprising the steps of:

(a) providing a chromatographic strip having a first end and a second end;

(b) providing an absorbent pad at the first end of the chromatographic strip, wherein the absorbent pad is in capillary contact with the chromatographic strip;

(c) providing a buffer pad near the first end of the chromatographic strip, wherein the buffer pad is positioned near, but not in contact with the absorbent pad;

(d) providing a sample pad at or near the second end of the chromatographic strip; and

(e) providing one or test zones between the buffer pad and the sample pad.

69. The method of claim 68, further comprising the step of providing a conjugate pad positioned below the sample pad or in between the sample pad and the one or more test zones.