Sensor Chip and Sensor Chip Production Method

• US 20090257917A1
• Filed: 10/16/2006
• Published: 10/15/2009
• Est. Priority Date: 10/17/2005
• Status: Abandoned Application

• Current Assignee: National Institute of Advanced Industrial Science and Technology (Government of Japan), Sumitomo Electric Industries Limited (Sumitomo Corporation)
• Original Assignee: National Institute of Advanced Industrial Science and Technology (Government of Japan), Sumitomo Electric Industries Limited (Sumitomo Corporation)
• Inventors: Nakamura, Hideaki, Gotoh, Masao, Karube, Isao, Ishikawa, Tomoko, Hosoya, Toshifumi, Kaimori, Shingo, Ichino, Moriyasu
• Application Number:
  US12/083,694
• Publication Number:
  US 20090257917A1
• Time in Patent Office: Days
• US Class Current: 422/68.100
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10T 156/1051 by folding

Abstract

It is intended to provide a sensor chip which has a small size and is easily produced and capable of determining quantities of at least two components of multiple samples rapidly, conveniently, and correctly as well as to provide a production method capable of producing the sensor chip easily and with high productivity.

A sensor chip includes a substrate, a cover layer, a spacer layer sandwiched between the substrate and the cover layer, multiple reaction portions disposed between the substrate and the cover layer, multiple detection units exposed in the hollow reaction portions, and a sample inlet communicated with the hollow reaction portions and a method for producing the sensor chip.

A sensor chip includes two substrates opposed to each other, a spacer layer sandwiched between the substrates, and multiple measurement units disposed between the substrates and including two or more hollow reaction portions that share one sample inlet opened on outer surfaces of the substrates and detection electrode units respectively exposed in the hollow reaction portions and a method for producing the sensor chip.

Claims

1. A sensor chip comprising:

a substrate,

a cover layer,

a spacer layer sandwiched between the substrate and the cover layer,

multiple hollow reaction portions disposed between the substrate and the cover layer,

multiple detection units exposed in the hollow reaction portions, wherein

a sample inlet is communicated with the hollow reaction portions.

2. The sensor chip according to claim 1, wherein

the substrate and the cover layer are formed from an identical material and have an identical thickness.

3. The sensor chip according to claim 1, wherein

the multiple hollow reaction portions are disposed to extend radially from the sample inlet.

4. The sensor chip according to claim 1, wherein

the sample inlet is disposed on an outer rim portion of the sensor chip.

5. The sensor chip according to claim 1, wherein

the sample inlet is disposed on a central portion of the substrate.

18. The sensor chip according to claim 1, which is a biosensor chip.

6. A sensor chip comprising:

two substrates opposed to each other,

a spacer layer sandwiched between the substrates, and

multiple measurement units disposed between the substrates, and including two or more hollow reaction portions that share one sample inlet opened on outer surfaces of the substrates and detection electrode units respectively exposed in the hollow reaction portions.

7. The sensor chip according to claim 6, wherein

each of the measurement units has two hollow reaction portions, and

the two hollow reaction portions are disposed to extend in directions reverse to each other from the sample inlet.

8. The sensor chip according to claim 6, wherein

the multiple measurement units are aligned along at least a direction of one straight line.

9. The sensor chip according to claim 8, further comprising:

multiple measurement units disposed in a direction orthogonal to the direction of one straight line.

10. The sensor chip according to claim 6, which is a biosensor chip.

11. A method for producing a sensor chip including a substrate, a cover layer, a spacer layer sandwiched between the substrate and the cover layer, a multiplicity of hollow reaction portions disposed between the substrate and the cover layer, a multiplicity of detection units exposed in the hollow reaction portions, wherein a sample inlet is communicated with the hollow reaction portions,

the method comprising:

a step of forming the multiple detection units on the substrate,

a step of forming the spacer layer having multiple grooves in which the detection units are respectively exposed and having one ends thereof disposed on a substantially identical position, and

a lamination step for covering the spacer layer with the cover layer.

12. The sensor chip production method according to claim 11, wherein

the substrate and the cover layer are formed from an identical material and have an identical thickness;

the multiple detection units are formed in a radial pattern on the cover layer;

the spacer layer having multiple grooves in which the detection units are respectively exposed and having one ends thereof disposed on a substantially identical position is formed; and

the lamination step is performed in such a manner that the ends of the grooves of the spacer layer formed on the substrate and the cover layer are overlapped with one another while other portions of the grooves are not overlapped.

13. The sensor chip production method according to claim 12, wherein

the substrate and the cover layer are formed from one substrate sheet, and

the lamination step is performed by folding the substrate sheet at a folding line at which the substrate sheet is substantially bisected.

14. The sensor chip production method according to claim 11, wherein

a reagent is coated on the groove before the lamination step.

15. A method for producing a sensor chip including two opposed substrates, a spacer layer sandwiched between the substrates, and multiple measurement unit including two or more hollow reaction portions sharing one sample inlet opened on outer surfaces of the substrates and detection electrode units respectively exposed in the hollow reaction portions,

the method comprising:

a step of forming the multiple detection electrode units on each of two parts that are sectioned by a folding line at which the substrate sheet is substantially bisected;

a step of covering the detection electrode units with a member forming the spacer layer and forming the multiple grooves in which the detection electrode units are respectively exposed in such a manner that one ends of the grooves are shared by the grooves or that one ends of the grooves are disposed line-symmetrically about a central axis which is the folding line; and

a step of folding the member to laminate surfaces of one part of the member and the other part of the member by the folding.

16. The sensor chip production method according to claim 15, wherein

the formation of the multiple detection electrode units are performed in such a manner that positions of the detection electrode units that are close to the folding line on one of the two parts sectioned by the folding line are different from positions of the detection electrode units that are close to the folding line on the other part.

17. The sensor chip production method according to claim 15, wherein

a reagent is coated on at least one of the grooves before the folding at the folding line.