METHODS AND COMPOSITIONS FOR PROVIDING TOLERANCE TO MULTIPLE HERBICIDES
First Claim
1. A method for controlling weeds in an area of cultivation comprising:
- a) evaluating environmental conditions in an area of cultivation;
b) selecting a combination of herbicides comprising at least an effective amount of a first and a second ALS inhibitor, wherein said effective amount of the combination controls weeds, and the effective amount of the first ALS inhibitor is not tolerated by the crop when applied alone when compared to a control crop that has been applied with the first ALS inhibitor, and the effective amount of the second ALS inhibitor is sufficient to produce a safening effect, wherein said safening effect provides an increase in crop tolerance upon the application of the first and the second ALS inhibitor when compared to the crop tolerance when the first ALS inhibitor is applied alone; and
,c) applying the combination of herbicides to a crop, crop part, seed or an area of cultivation of said crop, wherein the crop comprises a plant havingi) a first polynucleotide encoding a polypeptide that can confer tolerance to glyphosate operably linked to a promoter active in said plant; and
,ii) a second polynucleotide encoding an ALS inhibitor-tolerant polypeptide operably linked to a promoter active in said plant.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.
-
Citations
16 Claims
-
1. A method for controlling weeds in an area of cultivation comprising:
-
a) evaluating environmental conditions in an area of cultivation; b) selecting a combination of herbicides comprising at least an effective amount of a first and a second ALS inhibitor, wherein said effective amount of the combination controls weeds, and the effective amount of the first ALS inhibitor is not tolerated by the crop when applied alone when compared to a control crop that has been applied with the first ALS inhibitor, and the effective amount of the second ALS inhibitor is sufficient to produce a safening effect, wherein said safening effect provides an increase in crop tolerance upon the application of the first and the second ALS inhibitor when compared to the crop tolerance when the first ALS inhibitor is applied alone; and
,c) applying the combination of herbicides to a crop, crop part, seed or an area of cultivation of said crop, wherein the crop comprises a plant having i) a first polynucleotide encoding a polypeptide that can confer tolerance to glyphosate operably linked to a promoter active in said plant; and
,ii) a second polynucleotide encoding an ALS inhibitor-tolerant polypeptide operably linked to a promoter active in said plant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
Specification