MULTIFUNCTIONAL POLYMERIC TISSUE COATINGS
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Abstract
Compositions for coating biological and non-biological surfaces, which minimize or prevent cell-cell contact and tissue adhesion, and methods of preparation and use thereof are disclosed. Embodiments include polyethylene glycol/polylysine (PEG/PLL) block or comb-type copolymers with high molecular weight PLL (greater than 1000, more preferably greater than 100,000); PEG/PLL copolymers in which the PLL is a dendrimer which is attached to one end of the PEG; and multilayer compositions including alternating layers of polycationic and polyanionic materials. The multi-layer polymeric material is formed by the ionic interactions of a polycation and a polyanion. The molecular weights of the individual materials are selected such that the PEG portion of the copolymer inhibits cellular interactions, and the PLL portion adheres well to tissues. The compositions and methods are useful, for example, in inhibiting formation of post-surgical adhesions, protecting damaged blood vessels from thrombosis and restenosis, and decreasing the extent of metastasis of attachment-dependent tumor cells. The compositions and methods are also useful for coating non-biological surfaces such as metallic surfaces.
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Citations
63 Claims
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1-3. -3. (canceled)
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8-10. -10. (canceled)
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12-40. -40. (canceled)
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41. A method for coating a non-biological surface comprising applying to the surface a two-domain block copolymer
wherein the first domain comprises a non-ionic block, and wherein the second domain comprises a polycationic block, wherein the surface has a net anionic charge at physiologic pH, and wherein the polycationic block forms ionic bonds with the surface.
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43. (canceled)
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45-47. -47. (canceled)
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48. A composition comprising a dendritic copolymer, comprising
a polycationic dendrimer with a molecular weight sufficient to provide at least 8 cationic charges, and a non-ionic polymer covalently linked to the dendrimer, wherein the composition is in a form suitable for application to a biological or non-biological surface.
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54. A coating on a non-biological surface, comprising a two-domain block copolymer,
wherein the first domain comprises a non-ionic block, and wherein the second domain comprises a polycationic block, wherein the surface has a net anionic charge at physiologic pH, and wherein the polycationic block forms ionic bonds with the surface.
Specification