FUNCTIONAL NUCLEIC ACIDS FOR BIOLOGICAL SEQUESTRATION
First Claim
1. An expression vector comprising:
- a chimeric gene encoding selective nucleic acid ligands within a non-coding nucleic acid, operatively linked to a functional promoter, wherein said expression vector when transfected into a host transcribes said chimeric gene, and wherein said gene product is capable of binding to or altering at least one target molecule.
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Abstract
The present invention generally relates to methods of improving the removal and/or treatment of substances in bulk volumes, particularly to methods of improving the removal and/or treatment of contaminants in bulk volumes by nucleic acid interaction and by including such nucleic acid interactions in organisms. The present invention further relates to methods for generating and/or improving the interaction of nucleic acids with substances for removal and/or treatment. Bulk volumes may generally refer to any volume of substance wherein the removal and/or treatment of substances therein may occur. Nucleic acids may be utilized to bind and/or catalytically interact with substances in the bulk volume. Further, the nucleic acids may be included in an organism for sequestering substances within cells.
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Citations
30 Claims
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1. An expression vector comprising:
a chimeric gene encoding selective nucleic acid ligands within a non-coding nucleic acid, operatively linked to a functional promoter, wherein said expression vector when transfected into a host transcribes said chimeric gene, and wherein said gene product is capable of binding to or altering at least one target molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. An isolated cell comprising:
at least one nucleic acid ligand sequence incorporated into a genomic DNA encoding a non-coding nucleic acid, wherein said nucleic acid ligand sequence binds to or catalytically alters a target molecule. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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20. A method for sequestering within a cell a plurality of target molecules, present in a bulk volume comprising:
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generating a library of nucleic acid ligand sequences capable of binding to said target molecules; incorporating said nucleic acid ligand sequences into at least one non-coding nucleic acid within a cell; culturing said cell to achieve a cell population; contacting said cell population with said bulk volume; and separating said cell population from said bulk volume. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27)
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28. A method for bioremediation of at least one contaminant present in a bulk volume comprising:
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generating a library of nucleic acid ligand sequences capable of binding to or altering said at least one contaminant; incorporating said nucleic acid ligand sequences in at least one non-coding nucleic acid in a cell; culturing said cell to achieve a cell population; contacting said cell population with said bulk volume; and
,separating said cell population from said bulk volume.
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- 29. The method of claim 29, wherein said contaminants are at least one of an inorganic molecule, an organic molecule, a toxin, a protein, a peptide, and a viral particle.
Specification