Hemagglutinin Polypeptides, and Reagents and Methods Relating Thereto
First Claim
Patent Images
1. An engineered binding agent that binds to umbrella-topology glycans.
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0 Petitions
Accused Products
Abstract
The present invention provides a system for analyzing interactions between glycans and interaction partners that bind to them. The present invention also provides HA polypeptides that bind to umbrella-topology glycans, and reagents and methods relating thereto.
42 Citations
156 Claims
- 1. An engineered binding agent that binds to umbrella-topology glycans.
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16. An isolated binding agent that binds to umbrella-topology glycans other than, which binding agent is not an H1 protein from any of the strains:
- A/South Carolina/1/1918;
A/Puerto Rico/8/1934;
A/Taiwan/1/1986;
A/Texas/36/1991;
A/Beijing/262/1995;
A/Johannesburg/92/1996;
A/New Caledonia/20/1999;
A/Solomon Islands/3/2006, or an H2 protein from any of the strains;
A/Japan/305+/1957;
A/Singapore/1/1957;
A/Taiwan 1/1964;
A/Taiwan 1/1967, or an H3 protein from any of the strains;
A/Aichi/2/1968;
A/Phillipines/2/1982;
A/Mississippi/1/1985;
A/Leningrad/360/1986;
A/Sichuan/2/1987;
A/Shanghai/11/1987;
A/Beijing/353/1989;
A/Shandong/9/1993;
A/Johannesburg/33/1994;
A/Nanchang/813/1995;
A/Sydney/5/1997;
A/Moscow/10/1999;
A/Panama/2007/1999;
A/Wyoming/3/2003;
A/Oklahoma/323/2003;
A/California/7/2004;
A/Wisconsin/65/2005.
- A/South Carolina/1/1918;
- 17. A characteristic portion of an engineered binding agent that binds to umbrella-topology glycans.
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18. A characteristic portion of a binding agent, which binding agent is not an H1 protein from any of the strains:
- A/South Carolina/1/1918;
A/Puerto Rico/8/1934;
A/Taiwan/1/1986;
A/Texas/36/1991;
A/Beijing/262/1995;
A/Johannesburg/92/1996;
A/New Caledonia/20/1999;
A/Solomon Islands/3/2006, or an H2 protein from any of the strains;
A/Japan/305+/1957;
A/Singapore/1/1957;
A/Taiwan/1/1964;
A/Taiwan/1/1967, or an H3 protein from any of the strains;
A/Aichi/2/1968;
A/Phillipines/2/1982;
A/Mississippi/1/1985;
A/Leningrad/360/1986;
A/Sichuan/2/1987;
A/Shanghai/11/1987;
A/Beiging/353/1989;
A/Shandong/9/1993;
A/Johannesburg/33/1994;
A/Nanchang/813/1995;
A/Sydney/5/1997;
A/Moscow/10/1999;
A/Panama/2007/1999;
A/Fujian/411/2002;
A/Wyoming/3/2003;
A/Oklahoma/323/2003;
A/California/7/2004;
A/Wisconsin/65/2005, wherein the characteristic portion binds to umbrella-topology glycans.
- A/South Carolina/1/1918;
- 28. An antibody that binds to an engineered binding agent that binds to umbrella-topology glycans.
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29. An antibody that binds to a binding agent, which binding agent is not an H1 protein from any of the strains:
- A/South Carolina/1/1918;
A/Puerto Rico/8/1934;
A/Taiwan/1/1986;
A/Texas/36/1991;
A/Beijing/262/1995;
A/Johannesburg/92/1996;
A/New Caledonia/20/1999;
A/Solomon Islands/3/2006, or an H2 protein from any of the strains;
A/Japan/305+/1957;
A/Singapore/1/1957;
A/Taiwan/1/1964;
A/Taiwan/1/1967, or an H3 protein from any of the strains;
A/Aichi/2/1968;
A/Phillipines/2/1982;
A/Mississippi/1/1985;
A/Leningrad/360/1986;
A/Sichuan/2/1987;
A/Shanghai/11/1987;
A/Beiging/353/1989;
A/Shandong/9/1993;
A/Johannesburg/33/1994;
A/Nanchang/813/1995;
A/Sydney/5/1997;
A/Moscow/10/1999;
A/Panama/2007/1999;
A/Fujian/411/2002;
A/Wyoming/3/2003;
A/Oklahoma/323/2003;
A/California/7/2004;
A/Wisconsin/65/2005, wherein the binding agent binds to umbrella-topology glycans.
- A/South Carolina/1/1918;
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32. A viral particle including an engineered binding agent that binds to umbrella-topology glycans.
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33. A method of treating influenza infection by administering a composition comprising an engineered binding agent that binds to umbrella-topology glycans, a polypeptide comprising a characteristic fragment of an engineered binding agent that binds to umbrella-topology glycans, an antibody that binds to an engineered binding agent that binds to umbrella-topology glycans, or characteristic portion thereof, a nucleic acid that encodes an engineered binding agent that binds to umbrella-topology glycans or characteristic portion thereof, or combinations thereof.
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34. A glycan array comprising glycan structures of at least about 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% 95%, or more of glycans found on HA receptors in human upper respiratory tract tissues.
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37. A method of any of, inhibiting, in a subject, binding of an influenza virus to a hemagglutinin receptor having an umbrella topology, minimizing the risk of infection of a subject by an influenza virus which binds a hemagglutinin receptor having an umbrella topology, or treating a subject, comprising:
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identifying a subject susceptible to or suffering from infection by an influenza virus (optionally on the basis of need for blocking an influenza virus which binds a hemagglutinin receptor having an umbrella topology, or on the basis of being at risk for infection by an influenza virus which binds a hemagglutinin receptor having an umbrella topology); optionally, selecting a binding agent on the basis of the binding agent being able to bind a hemagglutinin receptor having umbrella topology glycans; optionally, providing a binding agent; administering an effective amount of the binding agent to the subject, so that binding by the virus to hemagglutinin receptors having umbrella topology glycans is reduced, the risk is minimized, or the patient is treated. - View Dependent Claims (38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108)
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109. A method of selecting a subject for treatment with a blocking agent comprising:
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identifying a subject suffering from or susceptible to influenza infection, or optionally, identifying a subject on the basis of need for blocking an HA receptor having an umbrella topology glycan or on the basis of being at risk for infection by an influenza virus which binds an HA receptor having an umbrella topology glycan; administering the blocking agent to the subject or selecting a dosage of the blocking agent for the subject; thereby selecting a subject for treatment with a bl-ocking agent. - View Dependent Claims (110, 111, 112, 113, 114, 115)
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116. A method of selecting a patient for treatment with blocking agent comprising:
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optionally, identifying a subject suffering from or susceptible to influenza infection; determining if the subject has antibodies that react with a blocking agent; if the subject lacks antibodies to the a blocking agent, selecting the subject for treatment, thereby selecting a patient for treatment with a blocking agent. - View Dependent Claims (117, 118, 119, 120, 121)
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122. A method of selecting a dosage of a blocking agent, comprising:
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optionally, identifying a subject suffering from or susceptible to influenza infection, e.g., identifying a subject on the basis of need for blocking an HA receptor having an umbrella topology glycan or on the basis of being at risk for infection by an influenza virus which binds an HA receptor having an umbrella topology glycan; determining if the subject has antibodies that react with a blocking agent; selecting a subject or dose based on the determination, thereby selecting a dosage for treatment with a blocking agent. - View Dependent Claims (123, 124, 125, 126, 127)
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128. A method of selecting a drug for administration to a subject comprising:
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identifying a subject suffering from or susceptible to influenza infection, or optionally, identifying a subject on the basis of need for blocking an HA receptor having an umbrella topology glycan or on the basis of being at risk for infection by an influenza virus which binds an HA receptor having an umbrella topology glycan; selecting a blocking agent on the basis that it blocks an UTHAr; optionally, providing a unit dose of a blocking agent to the subject or to a health care provider for administration to the subject. thereby selecting a drug for administration to a subject. - View Dependent Claims (129, 130, 131, 132, 133, 134)
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135. A method of evaluating a test binding agent, e.g., a polypeptide, e.g., naturally occurring or mutant HA, or an antibody, e.g., for use in minimizing risk of infection with by avian influenza comprising,
determining the ability of the agent to bind, e.g., specifically bind, to a HA having an umbrella topology glycan, thereby evaluating the agent.
- 136. A device containing a blocking agent and configured to administer a dose, e.g., a metered dose, of the blocking agent, to the respiratory tract of a subject.
- 139. A kit comprising an initial unit dose and a subsequent unit dose of a binding agent, e.g., an HA polypeptide, a LSBA, an UTBA, an UTSBA, wherein the initial unit dose is greater than the subsequent unit dose or where the two doses are equal.
- 141. A kit comprising at least one component of a delivery device, e.g., an inhaler and a dose of an of a binding agent.
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142. A kit comprising a dose of an of a binding agent.
- 144. A pharmaceutical composition comprising a binding agent.
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155. A pharmaceutical composition comprising a first binding agent and a second binding agent.
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156. A pharmaceutical compositions comprising an umbrella topology binding agent and a cone topology binding agent, e.g., a recombinant HA or fragment thereof which binds an HA receptor having a cone topology glycan, e.g., a 2-6 glycan having a cone topology.
Specification