Culture System and Method for Propagation of Human Blastocyst-Derived Stem Cells
First Claim
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1. A culture system for propagation of human blastocyst-derived stem (hBS) cells comprisingi) human feeder cells at a density of at least 50,000 cells/cm2,ii) one or more dissociation agents for dissociation of hBS cell colonies into a single cell suspension, andiii) a supportive culture medium.
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Abstract
The present invention relates to a culture system for and a method for propagation of human blastocyst-derived stem cells (hBS cells) upon enzymatic dissociation into a single cell suspension. The culture system for propagation of human blastocyst-derived stem (hBS) cells comprises
- i) human feeder cells at a density of at least 50,000 cells/cm2,
- ii) one or more dissociation agents for dissociation of hBS cell colonies into a single cell suspension, and
- iii) a supportive culture medium,
which culture system makes it possible to propagate hBS cells by dissociation of hBS cell colonies into a single cell suspension at each consecutive passage for an extended time period, while maintaining the significant characteristics of hBS cells.
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Citations
61 Claims
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1. A culture system for propagation of human blastocyst-derived stem (hBS) cells comprising
i) human feeder cells at a density of at least 50,000 cells/cm2, ii) one or more dissociation agents for dissociation of hBS cell colonies into a single cell suspension, and iii) a supportive culture medium.
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53-57. -57. (canceled)
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59. (canceled)
Specification