PLURIPOTENT CELLS
First Claim
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1. A method for deriving a population of cells comprising cells expressing pluripotency markers comprising the steps of:
- a. Obtaining cells, andb. Culturing the cells under hypoxic conditions, on a tissue culture substrate that is not pre-treated with a protein or an extracellular matrix prior to culturing the cells.
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Abstract
The present invention is directed to pluripotent cells that can be readily expanded in culture on tissue culture substrate that is not pre-treated with protein or an extracellular matrix, and do not require a feeder cell line. The present invention also provides methods to derive the pluripotent cell line from human embryonic stem cells.
89 Citations
83 Claims
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1. A method for deriving a population of cells comprising cells expressing pluripotency markers comprising the steps of:
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a. Obtaining cells, and b. Culturing the cells under hypoxic conditions, on a tissue culture substrate that is not pre-treated with a protein or an extracellular matrix prior to culturing the cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method for deriving a population of cells comprising cells expressing pluripotency markers, comprising the steps of:
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a. Culturing human embryonic stem cells, b. Differentiating the human embryonic stem cells into cells expressing markers characteristic of definitive endoderm cells, and c. Removing the cells, and subsequently culturing them under hypoxic conditions, on a tissue culture substrate that is not pre-treated with a protein or an extracellular matrix prior to culturing the cells. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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51. A method for deriving a population of cells comprising cells expressing pluripotency markers comprising the steps of:
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a. Culturing human embryonic stem cells, and b. Removing the cells, and subsequently culturing them under hypoxic conditions, on a tissue culture substrate that is not pre-treated with a protein or an extracellular matrix prior to culturing the cells. - View Dependent Claims (52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73)
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74. A method to expand cells expressing markers characteristic of the definitive endoderm lineage, comprising the steps of:
- culturing the cells under hypoxic conditions, on a tissue culture substrate that is not pre-treated with a protein or an extracellular matrix.
- View Dependent Claims (75, 76, 77, 78, 79, 80, 81, 82, 83)
Specification