Compositions comprising a linked acceptor moiety

• US 20090270602A1
• Filed: 06/30/2003
• Published: 10/29/2009
• Est. Priority Date: 03/24/1998
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Boston Probes, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Coull, James M., Gildea, Brian D., Hyldig-Nielsen, Jens J.
• Granted Patent: US 7,816,501 B2
• Time in Patent Office: Days
• US Class Current: 536/24.300
• CPC Class Codes: C07K 14/003 Peptide-nucleic acids (PNAs)
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6851 Quantitative amplification
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels

Abstract

This invention is directed to compositions comprising a linked acceptor moiety.

Claims

1. A non-nucleic acid polymer bearing at least one linked acceptor moiety but not a linked donor moiety.

2. The non-nucleic acid polymer of claim 1, wherein the polymer is a PNA.

3. The non-nucleic acid polymer of claim 2, wherein PNA subunits of the polymer have the formula:

4. The non-nucleic acid polymer of claim 3, wherein each PNA subunit consists of a naturally occurring nucleobase attached to the aza nitrogen of a N-[2-(aminoethyl)]glycine backbone through a methylene carbonyl linkage.

5. The non-nucleic acid polymer of claim 1, wherein the acceptor moiety is dabcyl.

6. The non-nucleic acid polymer of claim 5, wherein the quencher moiety is dabcyl.

7. The non-nucleic acid polymer of claim 2, wherein the acceptor moiety is dabcyl.

8. The non-nucleic acid polymer of claim 7, wherein the quencher moiety is dabcyl.