Compositions And Methods For Producing Fermentation Products And Residuals
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Accused Products
Abstract
The present invention provides compositions and methods designed to increase value output of a fermentation reaction. In particular, the present invention provides a business method of increasing value output of a fermentation plant. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microoganism.
58 Citations
52 Claims
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1-32. -32. (canceled)
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33. A fermentation residual comprising genetically modified yeast cells and distillers solubles from the fermentation, wherein:
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(a) the yeast cells comprise a recombinant expression vector comprising an exogenous nucleotide sequence encoding a polypeptide and a regulatory sequence that controls the expression of the polypeptide; (b) said fermentation residual is the product of an anaerobic, alcohol producing fermentation reaction; (c) said fermentation residual comprises a nutrient selected from the group consisting of an amino acid, a cofactor, a hormone, a protein, a vitamin and a lipid; and (d) the expression of said exogenous nucleotide sequence results in increased production of said polypeptide and said nutrient and in enhanced concentration of said nutrient in the fermentation residual compared with the concentration of said nutrient in a fermentation residual produced by unmodified, corresponding yeast cells when used in said anaerobic, alcohol producing fermentation reaction, whereby the residual has enhanced nutritional value. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42)
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43. A complete animal feed for mammals, poultry or aquaculture comprising a fermentation residual wherein the fermentation residual comprises genetically modified yeast cells and distillers solubles from the fermentation, wherein:
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(a) the yeast cells comprise a recombinant expression vector comprising an exogenous nucleotide sequence encoding a polypeptide and a regulatory sequence that controls the expression of the polypeptide; (b) said fermentation residual is the product of an anaerobic, alcohol producing fermentation reaction; (c) said fermentation residual comprises a nutrient selected from the group consisting of an amino acid, a cofactor, a hormone, a protein, a vitamin and a lipid; and (d) the expression of said exogenous nucleotide sequence results in increased production of said polypeptide and said nutrient and in enhanced concentration of said nutrient in the fermentation residual compared with the concentration of said nutrient in a fermentation residual produced by unmodified, corresponding yeast cells when used in said anaerobic, alcohol producing fermentation reaction, whereby the residual has enhanced nutritional value. - View Dependent Claims (44, 45, 46, 47, 48, 49, 50, 51, 52)
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Specification