CELLULAR EXTRACTS

• US 20090274770A1
• Filed: 05/07/2009
• Published: 11/05/2009
• Est. Priority Date: 05/11/2006
• Status: Active Grant

• Current Assignee: Regenics AS
• Original Assignee: Regenics AS
• Inventors: Remmereit, Jan, Gammelsaeter, Runhild
• Granted Patent: US 8,877,253 B2
• Time in Patent Office: Days
• US Class Current: 424/581
• CPC Class Codes: A61K 2800/805 Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
• CPC Class Codes: A61K 35/60 Fish, e.g. seahorses; Fish eggs
• CPC Class Codes: A61K 35/65 Amphibians, e.g. toads, frogs, salamanders or newts
• CPC Class Codes: A61K 8/987 of species other than mammals or birds
• CPC Class Codes: A61K 9/06 Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels composition of ointments, creams or gels A61K47/00
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/12 Keratolytics, e.g. wart or anti-corn preparations
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations

Abstract

The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. This invention relates to the use of cells and cellular extracts in rejuvenation and healing technologies thereby improving healing and regeneration of all bodily tissues and organs. The present invention relates to compositions and methods of managing, preventing, and treating scars. The invention also relates to prevention of deterioration, damage and malfunction of cells and tissues, and to promote, improve or exceed cellular function in order to promote, improve and exceed appearance, vitality and health. In some embodiments, the invention relates to compositions of cells, eggs, cell extracts, egg extracts, and extract components such as purified nucleic acids, polypeptides, lipids, carbohydrates or other natural products.

Claims

1. A composition comprising:

an egg cellular extract; and

a purified active compound.

2. The composition of claim 1, wherein said purified active compound is selected from the group consisting of a small molecule pharmaceutical compound and a pharmaceutical protein.

3. The composition of claim 1, wherein said egg cellular extract is selected from the group consisting of an extract of an activated fish egg, an unactivated fish egg, an activated amphibian egg and an unactivated amphibian egg.

4. The composition of claim 1, wherein said egg extract is from a fertilized egg.

5. The composition of claim 4, wherein said egg extract if from an egg selected from the group consisting of an amphibian egg and a fish egg.

6. The composition of claim 1, wherein said composition is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.

7. The composition of claim 1, wherein said egg extract is a cytoplasmic extract.

8. A method of increasing the penetration of an active compound into the skin of a subject comprising:

a) providing a composition according to claim 1; and

b) topically applying said composition to said skin of said subject so that said active compound is absorbed into the skin of said subject.

9. A method of increasing collagen protein production and/or collagen gene expression by the skin of a subject, comprising:

providing a composition comprising an egg extract; and

applying said egg extract to the skin of said subject under conditions such that collagen protein production and/or collagen gene expression is increased at the site of application.

10. The method of claim 9, wherein said composition is applied to a wound in the skin of said subject.

11. The method of claim 9, wherein said egg cellular extract is selected from the group consisting of an extract of an activated fish egg, an unactivated fish egg, an activated amphibian egg and an unactivated amphibian egg.

12. The method of claim 9, wherein said egg extract is from a fertilized egg.

13. The method of claim 12, wherein said egg extract if from an egg selected from the group consisting of an amphibian egg and a fish egg.

14. The method of claim 9, wherein said composition is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.

15. The method of claim 9, wherein said egg extract is a cytoplasmic extract.

16. A method of increasing cell proliferation in the skin of a subject, comprising:

providing a composition comprising an egg extract; and

applying said egg extract to the skin of said subject under conditions such that cell proliferation is increased at the site of application.

17. The method of claim 16, wherein said composition is applied to a wound in the skin of said subject.

18. A method of preparing extracts from amphibian of fish eggs comprising:

a) providing eggs from selected from the group consisting of amphibian and fish eggs;

b) treating said eggs to prevent growth of bacteria during the further processing of said eggs.

19. The method of claim 18, wherein said eggs are treated with a bactericidal or bacteristatic composition.

20. The method of claim 19, wherein said composition comprises iodine.

21. A method of making extracts from amphibian of fish eggs comprising:

a) providing eggs from selected from the group consisting of amphibian and fish eggs;

b) processing said eggs to provide a cytoplasmic fraction from said eggs;

c) treating said cytoplasmic fraction to prevent the growth of bacteria.

22. The method of claim 21, wherein said eggs are selected from the group consisting of fertilized and unfertilized eggs.

23. The method of claim 21, wherein said processing comprising homogenization of said eggs followed by centrifugation to separate lipids and solids from said cytoplasmic fraction.

24. The method of claim 21, wherein said treating comprising heating said extract to a temperature of from about 50 C to about 90 C for a period of greater than one minute.

25. A composition comprising:

about 100 to 250 mg/ml fish egg protein in an aqueous solution;

at least one trace element selected from the group consisting of calcium, phosphorous, zinc, copper and iron;

at least one vitamin selected from the group consisting of vitamins A, E, riboflavin, niacin, B 6, calcium pantothenate and B 12; and

a lipid fraction comprising from about 60 to 80% unsaturated fatty acids;

wherein said composition has an osmolarity of from about 330 to 440 mOsm, a pH of from about 5.0 to 7.7, and density of from about 0.8 to 1.4 g/ml.

26. A method of increasing collagen protein production and/or collagen gene expression by the skin of a subject, comprising:

applying a composition comprising a fish egg cellular extract comprising about 50 to 500 mg/ml fish egg protein, from about one to six grams/100 grams extract fish egg lipids, an osmolarity of from about 330 to 440 mOsm, and a pH of from about 5.0 to 7.7 to the skin of said subject under conditions such that collagen protein production and/or collagen gene expression is increased at the site of application.

29. A method of improving a parameter of wound healing in a subject, comprising:

applying a composition comprising a fish egg cellular extract comprising about 50 to 500 mg/ml fish egg protein, from about one to six grams/100 grams extract fish egg lipids, an osmolarity of from about 330 to 440 mOsm, and a pH of from about 5.0 to 7.7 to a wound of said subject under conditions such that a parameter of wound healing is improved, wherein said parameter is selected from the group consisting of faster drying, faster reepithelialization, reduced inflammation, faster contraction, earlier remodeling, improved remodeling, reduction in scar tissue and improved visual appearance of the wound and combinations thereof.

30. The method of claim 43, wherein said composition is applied to a wound in the skin of said subject.

31. The method of claim 43, wherein said composition is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.

32. The method of claim 43, wherein said egg extract is a cytoplasmic extract.

33. A method of increasing expression of a gene in a tissue of a subject comprising contacting the tissue of the subject with an egg extract under conditions such that expression of a gene is increased, wherein said gene is selected from the group consisting of collagen 1, collagen 3, VEGF-B, VEGF-C, TGFβ

2, TGFβ

3, PDGF-A, PDGF-B, PDGF-D, IL-18, and fibronectin.

34. The method of claim 33, wherein said egg extract is a cytoplasmic extract of fish or amphibian eggs.

35. A method of decreasing expression of a gene in a tissue of a subject comprising contacting the tissue of the subject with an egg extract under conditions such that expression of a gene is decreased, wherein said gene is selected from the group consisting of a matrix metallopeptidase, TGFβ

1, VEGF-A, elastin, IL 1β

, and IL 12.

27. The method of claim 37, wherein said composition is applied to a wound in the skin of said subject.

28. The method of claim 37, wherein said composition is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.

36. The method of claim 35, wherein said egg extract is a cytoplasmic extract of fish or amphibian eggs.

37. The method of claim 35, wherein said matrix metallopeptidase (MMP) is selected from the group consisting of MMP 14, 16, 17, 19, 20, 23, 25 and 28.

38. A method of treating a subject with skin condition comprising contacting the skin of said subject with a fish or amphibian cytoplasmic egg extract in an effective amount, wherein said skin condition is selected from the group consisting of ulcers, psoriasis, calluses, moles, acne, rosacea, dermatitis, keratosis, basal cell carcinoma and squamous cell carcinoma.

39. The method of claim 38, wherein said fish or amphibian cytoplasmic cell extract is provided in a cream, gel, emulsion, ointment, spray, powder or lotion.