Novel Nucleoside or Nucleotide Derivative and Use Thereof
First Claim
1. A nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, which has a fluorescent dye attached either directly or through a linker to the 5-position of the base, wherein the fluorescent dye is selected from the group consisting of 5-carboxyfluorescein (5-FAM), 6-carboxyfluorescein (6-FAM), 5-carboxytetramethylrhodamine (5-TAMRA), 6-carboxytetramethylrhodamine (6-TAMRA), 5-(dimethylamino)naphthalene-1-sulfonic acid (DANSYL), 5-carboxy-2′
- ,4,4′
,5′
,7,7′
-hexachlorofluorescein (5-HEX), 6-carboxy-2′
,4,4′
,5′
,7,7′
-hexachlorofluorescein (6-HEX), 5-carboxy-2′
,4,7,7′
-tetrachlorofluorescein (5-TET), 6-carboxy-2′
,4,7,7′
-tetrachlorofluorescein (6-TET), 5-carboxy-X-rhodamine (5-ROX), and 6-carboxy-X-rhodamine (6-ROX).
1 Assignment
0 Petitions
Accused Products
Abstract
The object of the present invention is to provide a nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, as well as a method using the same.
In one embodiment, the nucleoside or nucleotide of the present invention has a fluorescent dye selected from the group consisting of 5-FAM, 6-FAM, 5-TAMRA, 6-TAMRA, DANSYL, 5-HEX, 6-HEX, 5-TET, 6-TET, 5-ROX and 6-ROX or a quencher dye selected from the group consisting of DABCYL, BHQ1 and BHQ2, which is attached either directly or through a linker to the 5-position of the above base.
-
Citations
15 Claims
-
1. A nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, which has a fluorescent dye attached either directly or through a linker to the 5-position of the base, wherein the fluorescent dye is selected from the group consisting of 5-carboxyfluorescein (5-FAM), 6-carboxyfluorescein (6-FAM), 5-carboxytetramethylrhodamine (5-TAMRA), 6-carboxytetramethylrhodamine (6-TAMRA), 5-(dimethylamino)naphthalene-1-sulfonic acid (DANSYL), 5-carboxy-2′
- ,4,4′
,5′
,7,7′
-hexachlorofluorescein (5-HEX), 6-carboxy-2′
,4,4′
,5′
,7,7′
-hexachlorofluorescein (6-HEX), 5-carboxy-2′
,4,7,7′
-tetrachlorofluorescein (5-TET), 6-carboxy-2′
,4,7,7′
-tetrachlorofluorescein (6-TET), 5-carboxy-X-rhodamine (5-ROX), and 6-carboxy-X-rhodamine (6-ROX). - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
- ,4,4′
-
2. A nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, which has a quencher dye attached either directly or through a linker to the 5-position of the base, wherein the quencher dye is selected from the group consisting of 4-(4-dimethylaminophenylazo)benzoic acid (DABCYL), N-methyl-N-[4-[2-methoxy-5-methyl-4-(2-nitro-4-methylphenylazo)phenylazo]phenyl]-4-aminobutyric acid (BHQ1) and N-methyl-N-[4-[2,5-dimethoxy-4-(4-nitrophenylazo)phenylazo]phenyl]-4-aminobutyric acid (BHQ2).
Specification