ADJUSTABLE MOLECULAR MOTOR MICROPOWER BIOSENSOR AND ITS APPLICATION

• US 20090275052A1
• Filed: 12/15/2005
• Published: 11/05/2009
• Est. Priority Date: 12/16/2004
• Status: Active Grant

• Current Assignee: Institute of Biophysics Chinese Academy of Sciences
• Original Assignee: Institute of Biophysics Chinese Academy of Sciences
• Inventors: Yue, Jiachang
• Granted Patent: US 7,785,774 B2
• Time in Patent Office: Days
• US Class Current: 435/7.500
• CPC Class Codes: C12Q 1/34 involving hydrolase
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals

Abstract

A regulable molecular motor micropower biosensor comprises: a rotary motor, an optical energy conversion device, a signal molecular output device, a power resource system, a protective layer, a supporting material, and a bilayer lipid membrane fixing material. The molecular motor micropower biosensor is used for detecting biological macromolecules, viral molecules, etc.

Claims

1. An adjustable molecular motor micropower biosensor comprising

a rotary motor:

F₀F₁-ATPase;

an optical energy conversion device consisting of a light reaction center (RC) and a cold light source;

an electron conversion device which is the same system as the optical energy conversion device;

a signal molecular output device consisting of light excitation and emission device and a fluorescence probe;

a power resource system, consisting of water, ATP, ADP, Pi, and visible light;

a protective layer, being a bilayer lipid membrane; and

a supporting material and a fixing material for the bilayer lipid membrane.

2. The molecular motor micropower biosensor of claim 1, wherein the fluorescence probe is Lipids-fluorescein labeled outside the membrane.

3. The molecular motor micropower biosensor of claim 1, wherein the fluorescence probe is fluorescein labeled inside the membrane.

4. The molecular motor micropower biosensor according to claim 1, wherein rotation of the molecular motor is driven by ATP hydrolysis or synthesis.

5. The molecular motor micropower biosensor according to claim 1, wherein the rotation of the molecular motor is driven by transmembrane electrochemical potential gradient.

6. The molecular motor micropower biosensor of claim 5, wherein the transmembrane electrochemical gradient is converted from light energy or chemical energy.

7. The molecular motor micropower biosensor according to claim 1, wherein a β

subunit of the F₀F₁-ATPase binds to the an antibody of the β

subunit (primary antibody), which optionally further binds to a secondary antibody (IgG which specifically recognizes the primary antibody), to regulate the rotation speed of the molecular motor.

9. A method for detecting a biological macromolecule or a viral molecule at a single molecule lever, comprising the steps of:

(1) combining the molecular motor micropower biosensor according to claim 1 with an antibody of a specific biological macromolecule or a viral molecule antigen;

(2) contacting the molecular motor micropower biosensor obtained from step 1 with a sample to be tested;

(3) comparing fluorescence intensity between the molecular motor micropower biosensor obtained from step 2 and a molecular motor micropower biosensor having not been contacted with a the sample to be tested, under light excitation.

10. The method of claim 9, wherein the molecular motor micropower biosensor binds to the antibody of the specific biological macromolecule or the viral molecule antigen through a β

subunit antibody-biotin-streptavidin antibiotin-biotin.

8. (canceled)