TISSUE ENGINEERED BLOOD VESSELS

• US 20090275129A1
• Filed: 04/30/2009
• Published: 11/05/2009
• Est. Priority Date: 04/30/2008
• Status: Abandoned Application

• Current Assignee: Cardinal Health Switzerland 515 GmbH (Cardinal Health, Inc.)
• Original Assignee: Ethicon Incorporated (Johnson & Johnson)
• Inventors: Yang, Chunlin, Fang, Carrie H., Chun, Iksoo, Cooper, Kevin, Gosiewska, Anna, Colter, David C., Seyda, Agnieszka, Dhanaraj, Sridevi
• Application Number:
  US12/432,994
• Publication Number:
  US 20090275129A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C12M 21/08 for producing artificial tissue or for ex-vivo cultivation of tissue prostheses A61F2/00, grafts A61L27/00
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 5/0691 Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels

Abstract

Compositions and methods of using tissue engineered blood vessels to repair and regenerate blood vessels of patients with vascular disease are disclosed.

Claims

1. A tubular construct for a tissue engineered blood vessel comprising a scaffold comprising poly(p-dioxanone) and one or more or of cells, cell sheets, cell lysate and minced tissue.

2. The tubular construct of claim 1 wherein the scaffold further comprises collagen.

3. The tubular construct of claim 1 wherein the cells are selected from the group consisting of endothelial cells, smooth muscle cells, fibrobasts, human umbilical cord derived cells and combinations thereof.

4. A method of making a tissue engineered blood vessel comprising the steps of:

a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);

b. Seeding said scaffold with one or more cells selected from the group consisting of endothelial cells, smooth muscle cells, fibroblasts and human umbilical cord-derived cells; and

c. Culturing the cell seeded scaffold in a bioreactor.

5. A method of making a tissue engineered blood vessel comprising the steps of:

a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);

b. Wrapping said scaffold with a human umbilical cord-derived cell sheet; and

c. Culturing the cell sheet wrapped scaffold in a bioreactor.

6. A method of making a tissue engineered blood vessel comprising the steps of:

a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);

b. Applying said scaffold with minced muscle tissue and minced endothelial tissue; and

c. Culturing the minced tissue scaffold in a bioreactor.

7. A method of making a tissue engineered blood vessel comprising the steps of:

a. Providing a tubular shaped scaffold comprising poly(p-dioxanone);

b. Applying said scaffold with cell lysate; and

then

c. Lyophilizing the scaffold.

8. The method of claim 7 further comprising the steps of:

a. applying one or more of cells and minced tissue to the cell lysate scaffold; and

b. culturing the cell lysate scaffold having one or more of cells and minced tissue in a bioreactor.

9. A method of making a tissue engineered blood vessel comprising the steps of:

a. Providing a scaffold comprising poly(p-dioxanone);

b. Seeding said scaffold sheet with cells;

c. Rolling said seeded sheet into a tube; and

d. Culturing the tube in a bioreactor.