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METHOD FOR PRODUCTION OF MAST CELLS FROM STEM CELLS

  • US 20090275131A1
  • Filed: 05/04/2009
  • Published: 11/05/2009
  • Est. Priority Date: 05/02/2008
  • Status: Active Grant
First Claim
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1. A method of preparing mast cells by culturing pluripotent cells in vitro comprising the steps of:

  • a) culturing the pluripotent cells under conditions that promote differentiation of the cells into hematopoietic progenitor cells or megakaryocytes, to provide a first cell population comprising hematopoietic precursors or megakaryocytes; and

    b) culturing the first cell population under conditions that promote the differentiation into mast cells, to provide a second cell population comprising mast cells;

    wherein the step (a) culturing step does not employ a co-culture with murine fetal liver-derived stromal cells as a feeder layer.

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