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Production of ungulates, preferably bovines that produce human immunoglobulins

  • US 20090276866A1
  • Filed: 05/05/2008
  • Published: 11/05/2009
  • Est. Priority Date: 11/19/1999
  • Status: Active Grant
First Claim
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1. A method for producing a cloned ungulate wherein the expression of both copies of a gene essential for B-cell and T-cell production has been knocked out, selected from the group consisting of Igα

  • , E2A, EBF, BSAP, rag-1, and rag-2, which comprises the following steps;

    (i) producing an ungulate cell wherein the expression of one or both copies of the Igα

    , E2A, EBF, BSAP, rag-1, and/or rag-2 gene has been eliminated by targeted disruption;

    (ii) fusing or inserting said donor cell or nucleus into an enucleated oocyte or blastomere, to produce an embryo;

    (iii) introducing said embryo into a female ungulate; and

    (iv) obtaining a cloned fetus or animal ungulate that expresses the genotype of the donor differentiated cell, in which the expression of one or both copies of the Igα

    , E2A, EBF, BSAP, rag-1, and/or rag-2 gene has been eliminated; and

    (v) optionally, mating said male or female ungulate with another ungulate wherein one copy of the rag-1 or rag-2 gene has been knocked out and selecting progeny wherein both copies of the Igα

    , E2A, EBF, BSAP, rag-1, or rag-2 genes have been knocked out.

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