PLURIPOTENT CELLS
First Claim
1. A plurality of isolated mammalian cells that are immunopositive for nestin and for a marker of pluripotent cells, wherein the cells are suspended in a culture medium that has a total calcium concentration of 0.03 to 0.15 mM, and wherein the cells continue to proliferate in an undifferentiated state for at least 4 months.
1 Assignment
0 Petitions
Accused Products
Abstract
Pluripotent cells that are immunopositive for both the neural progenitor marker nestin and a pluripotent cell marker are provided. The cells exhibit rapid doubling times and can be maintained in vitro for extended periods. Also provided are cell cultures containing the pluripotent cells, a method of transplanting human pluripotent cells to a host, and a method of reducing seizure activity in a subject. These pluripotent cells, when transplanted into the ventricle of a host animal, migrate to the site of damage and adopt a suitably corrective phenotype, resulting in both structural and functional restoration.
71 Citations
25 Claims
- 1. A plurality of isolated mammalian cells that are immunopositive for nestin and for a marker of pluripotent cells, wherein the cells are suspended in a culture medium that has a total calcium concentration of 0.03 to 0.15 mM, and wherein the cells continue to proliferate in an undifferentiated state for at least 4 months.
Specification