RAPID AND EFFICIENT CAPTURE OF DNA FROM SAMPLE WITHOUT USING CELL LYSING REAGENT
First Claim
1. A kit for detection of K-ras mutation in a biological sample, said kit comprising a diagnostic K-ras primer selected from the group consisting of:
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Accused Products
Abstract
Nucleic acids can be made available for amplification or other treatment after admixture of a sample with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. No surfactant or other cell lysing reagents are employed. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.
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Citations
13 Claims
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1. A kit for detection of K-ras mutation in a biological sample, said kit comprising a diagnostic K-ras primer selected from the group consisting of:
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2. The oligonucleotide TGAATATAAA CTTGTGGTAC CTGGAGC T <
- SEQ ID;
NO 1>
.
- SEQ ID;
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3. The oligonucleotide ATATAAACTT GTGGTAGTTC CAGCTGGT <
- SEQ ID;
NO 2>
.
- SEQ ID;
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4. The oligonucleotide GAATTAGCTG TATCGTCAAG GCACTC <
- SEQ ID;
NO 3>
.
- SEQ ID;
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5. The oligonucleotide TCAGCAAAGA CAAGACAGGT A <
- SEQ ID;
NO 4>
.
- SEQ ID;
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6. The oligonucleotide TATAGATGGT GAAACCTGTT TGTTGG <
- SEQ ID;
NO 5>
.
- SEQ ID;
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7. The oligonucleotide CTTGCTATTA TTGATGGCAA CCACACAGA <
- SEQ ID;
NO 6>
.
- SEQ ID;
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8. A K-ras diagnostic primer comprising the oligonucleotide TGAATATAAA CTTGTGGTAC CTGGAGC T<
- SEQ ID;
NO 1>
.
- SEQ ID;
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9. A K-ras diagnostic primer comprising the oligonucleotide ATATAAACTT GTGGTAGTTC CAGCTGGT <
- SEQ ID;
NO 2>
.
- SEQ ID;
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10. A K-ras diagnostic primer comprising the oligonucleotide GAATTAGCTG TATCGTCAAG GCACTC <
- SEQ ID;
NO 3>
.
- SEQ ID;
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11. A K-ras diagnostic primer comprising the oligonucleotide TCAGCAAAGA CAAGACAGGT A <
- SEQ ID;
NO 4>
.
- SEQ ID;
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12. A K-ras diagnostic primer comprising the oligonucleotide TATAGATGGT GAAACCTGTT TGTTGG <
- SEQ ID;
NO 5>
.
- SEQ ID;
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13. A K-ras diagnostic primer comprising the oligonucleotide CTTGCTATTA TTGATGGCAA CCACACAGA <
- SEQ ID;
NO 6>
.
- SEQ ID;
Specification
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- Resources
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Current AssigneeOrtho-Clinical Diagnostics Incorporated (The Carlyle Group LP (Corporate Private Equity))
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Original AssigneeOrtho-Clinical Diagnostics Incorporated (The Carlyle Group LP (Corporate Private Equity))
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InventorsBelly, Robert T., Sun, Jianbo
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Application NumberUS12/503,500Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/6CPC Class CodesC12N 15/1003 Extracting or separating nu...C12Q 1/6806 Preparing nucleic acids for...C12Q 1/6886 for cancer immunoassay for ...C12Q 2527/119 pHC12Q 2527/125 Specific component of sampl...C12Q 2600/156 Polymorphic or mutational m...C12Q 2600/16 Primer sets for multiplex a...
