GENETIC SEQUENCER INCORPORATING FLUORESCENCE MICROSCOPY
First Claim
1. In a genome sequencer, a system for transporting fluids comprising:
- a mixing chamber;
storage for a plurality of reagents;
a multi-port valve in fluid communication with the storage;
a multi-port pump in fluid communication with the multi-port valve and the mixing chamber, the multi-port pump operable to draw at least one reagent of the plurality of reagents from the storage via the at least one multi-port valve into the mixing chamber; and
a flow cell in fluid communication with the multi-port pump and the mixing chamber, wherein the multi-port pump is operable to draw a mixture comprising the at least one reagent from the mixing chamber into the flow cell.
1 Assignment
0 Petitions
Accused Products
Abstract
A fluorescence microscopy sequencer comprises a fluid transport subsystem in which reagents are pumped through a series of multi-port valves to a mixer or one or more flow cells, or directly into the flow cell(s). The one or more multi-port valves can be mounted upon a fluids manifold having syringe tubes mounted on the opposite side. Mounted on a movable support, the manifold may be brought into and out of fluid communication with a storage block comprising the plurality of reagents. In another embodiment, the sequencer comprises a beamsplitter indexer that facilitates the quick and reliable switching of filter cubes through use of a stepper motor. In yet another embodiment, a motion control system is provided in which an inertial reference is interposed between and directly coupled to a first and second axis of control, thereby minimizing any low structural resonant frequencies and enabling high performance (high frequency response) motion control.
6 Citations
26 Claims
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1. In a genome sequencer, a system for transporting fluids comprising:
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a mixing chamber; storage for a plurality of reagents; a multi-port valve in fluid communication with the storage; a multi-port pump in fluid communication with the multi-port valve and the mixing chamber, the multi-port pump operable to draw at least one reagent of the plurality of reagents from the storage via the at least one multi-port valve into the mixing chamber; and a flow cell in fluid communication with the multi-port pump and the mixing chamber, wherein the multi-port pump is operable to draw a mixture comprising the at least one reagent from the mixing chamber into the flow cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. In a genome sequencer, a system for transporting fluids comprising:
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a manifold; a multi-port valve mounted upon and in fluid communication with a first side of the manifold; a plurality of syringe tubes mounted upon and in fluid communication with a second side of the manifold, each of the plurality of syringe tubes in fluid communication with a corresponding port of the multi-port valve via the manifold; a storage block comprising a plurality of recesses for storage of reagents, each of the plurality of recesses aligned with a corresponding one of the plurality of syringe tubes; and a movable support supporting the manifold and the storage block such that the plurality of syringe tubes can be moved into and out of fluid communication with the plurality of recesses. - View Dependent Claims (11, 12)
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13. In a genome sequencer, a beamsplitter indexer comprising:
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a support member comprising an index indicator; a plurality of beamsplitters coupled to the support member; a stepper motor directly coupled to the support member; a sensor, positioned relative to the support member to detect presence of the index indicator; and a controller, in communication with the sensor and the stepper motor, operative to control the stepper motor at an initial position upon receiving an indication from the sensor of the presence of the index indicator. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. In a genome sequencer, a motion control system comprising:
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an inertial reference; an objective; a target platform; a first axis of control, directly coupled to the inertial reference and the objective, operable to control motion of the objective along a first axis; a second axis of control directly coupled to the inertial reference; and a third axis of control coupled to the second axis of control and the target platform, the second axis of control operable to control motion of the target platform along a second axis perpendicular to the first axis, and the third axis of control operable to control motion of the target platform along a third axis perpendicular to the first axis and the second axis. - View Dependent Claims (24, 25, 26)
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Specification