Epitope-Captured Antibody Display
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Accused Products
Abstract
Reagents and methods for detecting target proteins in a sample are provided. The reagents include a replicable genetic package, a protein displayed on an exterior surface of the package that is expressed from a heterologous nucleic acid borne by the package, and one or more antibodies complexed with the expressed protein and which have an open binding site for a target protein. Thus, a segment of the nucleic acid encodes for an epitope that is shared by the expressed polypeptide and the target protein. The reagents can be utilized individually or as part of a library or an array to bind target proteins within protein samples to form one or more complexes. By determining the sequence of the segment of the heterologous nucleic acid of a package within a complex, one can identify the target protein since the segment encodes for an epitope that is shared by the expressed and target proteins.
15 Citations
37 Claims
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1-20. -20. (canceled)
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21. An array of polypeptides, comprising
(a) a support; - and
(b) a plurality of polypeptides immobilized at different locations on the support, wherein there are at least 103 locations/cm2 on the support, each location having at least one of the plurality of polypeptides immobilized therein, and wherein polypeptides in at least some of the locations differ in amino acid sequence and/or another property from polypeptides in other locations. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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36. An array, comprising
(a) a support; - and
(b) a plurality of polypeptides immobilized to the support, at least some of the plurality of polypeptides complexed with a captured antibody of a package/antibody reagent, each package/antibody reagent comprising a replicable genetic package that displays a polypeptide that is complexed to the captured antibody. - View Dependent Claims (37)
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Specification