Methods, Devices, Kits and Compositions for Detecting Roundworm

• US 20090286228A1
• Filed: 05/18/2009
• Published: 11/19/2009
• Est. Priority Date: 05/19/2008
• Status: Active Grant

• Current Assignee: Monsanto Company (Bayer AG), IDEXX Laboratories Incorporated
• Original Assignee: Divergence, Inc. (Bayer AG), IDEXX Laboratories Incorporated
• Inventors: Flynn, Laurie A., Crawford, Michael, Elsemore, David Allen
• Granted Patent: US 7,993,862 B2
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: C07K 14/4354 from nematodes
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Abstract

Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a mammalian sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Claims

1. An isolated polypeptide comprising an amino acid sequence at least 80% identical to SEQ ID NOs:

3, 4, 5, 6 or 7.

3. The isolated polypeptide of claim 1 wherein the polypeptide comprises SEQ ID NOs:

3, 4, 5, 6 or 7.

4. The isolated polypeptide of claim 1 wherein the polypeptide has a sequence that is identical to SEQ ID NOs:

3, 4, 5, 6 or 7 except for conservative amino acid substitutions.

5. An isolated antibody wherein the antibody specifically binds to the polypeptide of claim 1.

9. An isolated antibody wherein the antibody specifically binds to a polypeptide that has an amino acid sequence that corresponds to claim 1, and wherein the antibody specifically binds roundworm antigen in a fecal sample obtained from a roundworm-infected mammal.

10. The isolated antibody of claim 9, wherein the mammal is further infected with one or more of hookworm, whipworm and heartworm and the antibody does not specifically bind to any antigen from the one or more of hookworm, whipworm, or heartworm that may be present in the fecal sample.

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11. An isolated polypeptide consisting of SEQ ID NO:

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7, or an antigenic portion thereof.

64. An isolated antibody wherein the antibody specifically binds to the polypeptide of claim 11.

68. An isolated antibody wherein the antibody specifically binds to a polypeptide that has an amino acid sequence that corresponds to claim 11, and wherein the antibody specifically binds roundworm antigen in a fecal sample obtained from a roundworm-infected mammal.

69. The isolated antibody of claim 68, wherein the mammal is further infected with one or more of hookworm, whipworm and heartworm and the antibody does not specifically bind to any antigen from the one or more of hookworm, whipworm, or heartworm that may be present in the fecal sample.

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13. A device for detecting the presence or absence of roundworm antigens from a sample;

the device comprising a solid support, wherein the solid support has immobilized thereon one or more antibodies that are capable of specifically binding to a polypeptide that has an amino acid sequence that corresponds to SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, or SEQ ID NO;

7, or an antigenic portion thereof.

23. The device of claim 13 wherein the device further includes one or more reagents for the detection of one or more of the group consisting of:

one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi, and one or more bacteria.

59. A kit for detection of one or more roundworm antigens in a mammalian sample, the kit comprising the device of claim 13, and one or more reagents sufficient for the detection of the one or more antigens.

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24. A method of detecting the presence or absence of one or more roundworm antigens in a sample, the method comprising:

(a) contacting a sample from a mammal with one or more antibodies that can specifically bind to a polypeptide having amino acid sequence that corresponds to SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, or SEQ ID NO;

7;

or antigenic portion thereof,

(b) forming antibody-polypeptide complexes in the presence of the polypeptides, if any, in the sample; and

(c) detecting the presence or absence of the antibody-polypeptide complexes, if any.

38. The method of claim 24 further comprising the step of contacting the sample with one or more reagents to detect one or more of the group consisting of:

one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi, and one or more bacteria.

39. The method of claim 38 wherein the reagents for the detection of any one or all of the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi and one or more bacteria are one or more antibodies or one or more antigens recognized by antibodies specific for the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi or one or more bacteria.

40. The method of claim 24 further comprising the step of determining the presence or absence of a nucleic acid from the roundworm.

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43. A method of diagnosing whether a mammal is infected with an intestinal roundworm infection, the method comprising the steps of:

(a) contacting a sample from a mammal with one or more antibodies that can specifically bind to a polypeptide having amino acid sequence that corresponds to SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, or SEQ ID NO;

7, or antigenic portion thereof,

(b) forming antibody-polypeptide complexes in the presence of the polypeptides, if any, in the sample;

(c) detecting the presence or absence of the antibody-polypeptide complexes, if any; and

(d) diagnosing the mammal as having the roundworm infection if a roundworm antibody-polypeptide complex is present.

54. The method of claim 43 further comprising the step of contacting the sample with one or more reagents to detect one or more of the group consisting of:

one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi, and one or more bacteria.

55. The method of claim 54 wherein the reagents for the detection of any one or all of the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi and the one or more bacteria are one or more antibodies or one or more antigens recognized by antibodies specific for one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi or one or more bacteria.

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