Methods, Devices, Kits and Compositions for Detecting Roundworm
First Claim
1. An isolated polypeptide comprising an amino acid sequence at least 80% identical to an amino acid sequence selected from SEQ ID NOs:
- 3, 4, 5, 6, 7, 11, or Copro6728.
3 Assignments
0 Petitions
Accused Products
Abstract
Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.
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Citations
71 Claims
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1. An isolated polypeptide comprising an amino acid sequence at least 80% identical to an amino acid sequence selected from SEQ ID NOs:
- 3, 4, 5, 6, 7, 11, or Copro6728.
- View Dependent Claims (5, 9, 10)
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11. An isolated polypeptide consisting of SEQ ID NO:
- 3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
11, or Copro6728, or an antigenic portion thereof. - View Dependent Claims (66, 70, 71)
- 3, SEQ ID NO;
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12. (canceled)
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13. A device for detecting the presence or absence of roundworm antigens from a sample;
- the device comprising a solid support, wherein the solid support has immobilized thereon one or more antibodies that are capable of specifically binding to a polypeptide that has an amino acid sequence that corresponds to SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
11, or Copro6728, or an antigenic portion thereof. - View Dependent Claims (17, 23, 59)
- the device comprising a solid support, wherein the solid support has immobilized thereon one or more antibodies that are capable of specifically binding to a polypeptide that has an amino acid sequence that corresponds to SEQ ID NO;
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24. A method of detecting the presence or absence of one or more roundworm antigens in a sample, the method comprising:
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(a) contacting a sample from a mammal with one or more antibodies that can specifically bind to a polypeptide having amino acid sequence that corresponds to SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
11, or Copro6728, or an antigenic portion thereof,(b) forming antibody-polypeptide complexes in the presence of the polypeptides if any, in the sample; and (c) detecting the presence or absence of the antbody-polypeptide complexes, if any. - View Dependent Claims (38, 39)
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43. A method of diagnosing whether a mammal is infected with an intestinal roundworm infection, the method comprising the steps of:
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(a) contacting a sample from a mammal with one or more antibodies that can specifically bind to a polypeptide having amino acid sequence that corresponds to SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
11, or Copro6728, or antigenic portion thereof,(b) forming antibody-polypeptide complexes in the presence of the polypeptides, if any, in the sample; (c) detecting the presence or absence of the antibody-polypeptide complexes, if any; and (d) diagnosing the mammal as having the roundworm infection if a roundworm antibody-polypeptide complex is present. - View Dependent Claims (54, 55)
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61. A purified Copro6728 polypeptide.
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62. A purified polypeptide selected from the group consisting of SEQ ID NO:
- 3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, and SEQ ID NO;
11.
- 3, SEQ ID NO;
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Specification