ENGINEERED LUCIFERASES
First Claim
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1. A modified luciferase comprising a selectivity for ATP over dATP that is at least three-fold greater than the selectivity for ATP over dATP of an unmodified luciferase from the same organism.
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Abstract
DNA sequencing techniques are important for a variety of research and diagnostic applications. Pyrosequencing is a “sequencing by synthesis” technique that makes use of luciferase. Modified luciferase enzymes and methods of DNA pyrosequencing are provided. Means of preparing and producing mutant luciferases that have enhanced selectivity for ATP or dATP are described.
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23 Claims
- 1. A modified luciferase comprising a selectivity for ATP over dATP that is at least three-fold greater than the selectivity for ATP over dATP of an unmodified luciferase from the same organism.
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