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Method and Compositions for Detection and Enumeration of Genetic Variations

  • US 20090286687A1
  • Filed: 01/29/2009
  • Published: 11/19/2009
  • Est. Priority Date: 07/05/2003
  • Status: Abandoned Application
First Claim
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1. A method for sequencing nucleic acids comprising:

  • (a) fragmenting genomic nucleic acid molecules to generate a plurality of fragmented nucleic acids;

    (b) delivering the fragmented nucleic acids into aqueous microreactors in a water-in-oil emulsion such that a plurality of aqueous microreactors comprise a single copy of a fragmented nucleic acid, a single bead capable of hybridizing to the fragmented nucleic acid, and amplification reaction solution containing reagents necessary to perform nucleic acid amplification;

    (c) amplifying the fragmented nucleic acids in the microreactors to form amplified copies of said nucleic acids and hybridizing the amplified copies to beads in the microreactors;

    (d) delivering the beads to an array of reaction chambers, wherein a plurality of the reaction chambers comprise no more than a single nucleic acid bound bead; and

    (e) performing a sequencing reaction simultaneously on a plurality of the reaction chambers.

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